Detailed information of TA system TA092923

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 960177..960921
• Replicon: chromosome
• Accession: NZ_CP026047
• Organism: Raoultella planticola strain FDAARGOS_64

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1Q8YX07
• Locus tag: MC50_RS04565
• Protein ID: WP_044349292.1
• Coordinates: 960544..960921 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MC50_RS04560
• Protein ID: WP_170960574.1
• Coordinates: 960177..960503 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC50_RS04530	956619..957251	-	633	WP_072024984.1	inovirus Gp2 family protein	-
MC50_RS04535	957792..958616	+	825	WP_044349280.1	DUF945 domain-containing protein	-
MC50_RS04540	958613..958870	+	258	WP_044349282.1	DUF905 domain-containing protein	-
MC50_RS04545	958950..959393	+	444	WP_047663501.1	antirestriction protein	-
MC50_RS04550	959405..959884	+	480	WP_044349286.1	DNA repair protein RadC	-
MC50_RS04555	959898..960119	+	222	WP_044349288.1	DUF987 domain-containing protein	-
MC50_RS04560	960177..960503	+	327	WP_170960574.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MC50_RS04565	960544..960921	+	378	WP_044349292.1	TA system toxin CbtA family protein	Toxin
MC50_RS04570	960918..961409	+	492	WP_044349294.1	hypothetical protein	-
MC50_RS04575	961538..961866	+	329	Protein_865	DUF4942 domain-containing protein	-
MC50_RS04585	962786..963247	-	462	Protein_866	alpha-glucosidase C-terminal domain-containing protein	-
MC50_RS04590	963252..963710	+	459	Protein_867	iron-containing redox enzyme family protein	-
MC50_RS04595	963711..964289	-	579	WP_047663502.1	TetR/AcrR family transcriptional regulator	-
MC50_RS04600	965176..965661	+	486	WP_032697582.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	mrkJ / mrkF / mrkC / mrkB / mrkA	919489..965661	46172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13849.76 Da
• Isoelectric Point: 6.9771

>T92923 WP_044349292.1 NZ_CP026047:960544-960921 [Raoultella planticola]
MKILSDSPTRAAPSRPSPVEIWQTLLTYLLDQHYGLTLNDTPFGHDGVIQEHIGTGITLCDAVNLIVEKYKLVRIDRHGF
SADTQSPLIGSIDILRARKETGLMTRHGYRAITDITTGKYSEAGQ

Nucleotide

• Length: 378 bp

>T92923 NZ_CP026047:960544-960921 [Raoultella planticola]
ATGAAAATATTATCAGACTCCCCCACACGGGCGGCGCCGTCGCGCCCGTCTCCCGTTGAAATCTGGCAGACTCTGTTGAC
GTACCTGCTGGATCAGCACTACGGTCTTACGCTTAACGACACACCGTTTGGCCACGATGGCGTGATCCAGGAGCATATTG
GCACCGGGATCACCCTGTGTGATGCGGTGAATCTTATCGTCGAAAAATACAAACTGGTCCGTATCGATCGTCACGGTTTC
AGCGCAGACACACAGTCACCGCTTATCGGCAGTATCGATATCCTGCGGGCCCGAAAAGAAACTGGGCTGATGACCCGCCA
TGGCTACAGAGCGATTACTGATATCACGACGGGTAAATACAGTGAGGCCGGGCAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12101.76 Da
• Isoelectric Point: 6.4639

>AT92923 WP_170960574.1 NZ_CP026047:960177-960503 [Raoultella planticola]
ISEPWWGLRRSVSPCFGARLVQEGNRLNYLADRANFDGQFCDADLRHLDQAFPVLMKQLELMLTSGELNPRHQHCVTLYA
KGLTCEADTLGSHGYLYLAIYTTPATAA

Nucleotide

• Length: 327 bp

>AT92923 NZ_CP026047:960177-960503 [Raoultella planticola]
ATCAGCGAACCCTGGTGGGGGCTCAGGCGCAGTGTCTCTCCGTGTTTTGGGGCCCGTCTGGTCCAGGAGGGGAATCGGCT
GAATTATCTGGCTGACCGTGCGAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTTACCAGCGGTGAACTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGTCTGACCTGCGAGGCCGATACCCTCGGCTCACACGGTTACCTTTACCTCGCGATTTACACAACGCCTGCTACTGC
CGCATAA

Structures

Toxin

• AlphaFold DB: A0A1Q8YX07