Detailed information of TA system TA092908

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2107416..2108070
• Replicon: chromosome
• Accession: NZ_CP026045
• Organism: Citrobacter freundii strain FDAARGOS_61

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: MC47_RS10425
• Protein ID: WP_043017506.1
• Coordinates: 2107663..2108070 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: MC47_RS10420
• Protein ID: WP_016154349.1
• Coordinates: 2107416..2107682 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC47_RS10390	2102619..2104052	-	1434	WP_043017510.1	6-phospho-beta-glucosidase BglA	-
MC47_RS10395	2104172..2104900	-	729	WP_008785744.1	MurR/RpiR family transcriptional regulator	-
MC47_RS10400	2104953..2105264	+	312	WP_047408909.1	N(4)-acetylcytidine aminohydrolase	-
MC47_RS10405	2105428..2106087	+	660	WP_043017508.1	hemolysin III family protein	-
MC47_RS10410	2106179..2107159	-	981	WP_047408911.1	tRNA-modifying protein YgfZ	-
MC47_RS10420	2107416..2107682	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
MC47_RS10425	2107663..2108070	+	408	WP_043017506.1	protein YgfX	Toxin
MC47_RS10430	2108170..2108691	-	522	WP_043017505.1	flavodoxin FldB	-
MC47_RS10435	2108805..2109701	+	897	WP_003825519.1	site-specific tyrosine recombinase XerD	-
MC47_RS10440	2109725..2110438	+	714	WP_043017504.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
MC47_RS10445	2110444..2112177	+	1734	WP_047408916.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15891.76 Da
• Isoelectric Point: 11.5692

>T92908 WP_043017506.1 NZ_CP026045:2107663-2108070 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMNGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDVGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T92908 NZ_CP026045:2107663-2108070 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGCTGCTCATTCATGGGCTGGTGGCGGT
ATTCATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGAAGCCAGCGCCGAATTAACGCCTGTCAGGGCGAGATTCGGCTGTTGATGAACGGTCGACTGCGCTGGCAAGGGCAA
GAGTGGTCTATTGTTAGCGCACCCTGGATGGTCAAAACGGGCATGATGCTGCGTTTGCGTTCTGATGTCGGAAAGCGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTGCGACGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT92908 WP_016154349.1 NZ_CP026045:2107416-2107682 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT92908 NZ_CP026045:2107416-2107682 [Citrobacter freundii]
ATGGATATTAACAACAAAGCGCGTATTCATTGGGCATGCCGTCGCGGTATGCGCGAGTTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATACGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TGTTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTCAGTTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: R8WMJ6