Detailed information of TA system TA092670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 21141..21811
• Replicon: plasmid pK2044
• Accession: NZ_CP026012
• Organism: Klebsiella pneumoniae strain K2044

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: C2D63_RS24995
• Protein ID: WP_004213072.1
• Coordinates: 21141..21584 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: C2D63_RS25000
• Protein ID: WP_004213073.1
• Coordinates: 21581..21811 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2D63_RS24960	16552..16827	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
C2D63_RS24965	16890..17381	+	492	WP_011251327.1	DM13 domain-containing protein	-
C2D63_RS24970	17430..18350	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
C2D63_RS24975	18441..18847	+	407	Protein_20	GAF domain-containing protein	-
C2D63_RS26560	19362..19997	-	636	Protein_21	response regulator	-
C2D63_RS24985	20414..20707	+	294	Protein_22	transposase	-
C2D63_RS24990	20711..21127	+	417	Protein_23	restriction endonuclease	-
C2D63_RS24995	21141..21584	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
C2D63_RS25000	21581..21811	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C2D63_RS25005	22419..23552	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
C2D63_RS25010	23568..23861	+	294	WP_004213076.1	hypothetical protein	-
C2D63_RS25015	23851..24057	-	207	WP_004213077.1	hypothetical protein	-
C2D63_RS25020	24409..24699	+	291	WP_004213078.1	hypothetical protein	-
C2D63_RS25025	24689..25588	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroB / iroC / iroD / iroN / rmpA	1..224152	224152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T92670 WP_004213072.1 NZ_CP026012:c21584-21141 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T92670 NZ_CP026012:c21584-21141 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT92670 WP_004213073.1 NZ_CP026012:c21811-21581 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT92670 NZ_CP026012:c21811-21581 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620