Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1637959..1638595 Replicon chromosome
Accession NZ_CP026010
Organism Bacillus subtilis strain ATCC 11774

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag BS11774_RS08560 Protein ID WP_003156187.1
Coordinates 1638245..1638595 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag BS11774_RS08555 Protein ID WP_003225183.1
Coordinates 1637959..1638240 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS11774_RS08535 1634318..1634917 - 600 WP_042977090.1 rhomboid family intramembrane serine protease -
BS11774_RS08540 1635012..1635377 + 366 WP_014475874.1 holo-ACP synthase -
BS11774_RS08545 1635543..1636559 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
BS11774_RS08550 1636674..1637843 + 1170 WP_015252766.1 alanine racemase -
BS11774_RS08555 1637959..1638240 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
BS11774_RS08560 1638245..1638595 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BS11774_RS08565 1638710..1639534 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
BS11774_RS08570 1639539..1639904 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
BS11774_RS08575 1639908..1640309 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
BS11774_RS08580 1640321..1641328 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
BS11774_RS08585 1641390..1641719 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
BS11774_RS08590 1641716..1642198 + 483 WP_042977088.1 anti-sigma B factor RsbW -
BS11774_RS08595 1642164..1642952 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
BS11774_RS08600 1642952..1643551 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T92651 WP_003156187.1 NZ_CP026010:1638245-1638595 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T92651 NZ_CP026010:1638245-1638595 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT92651 WP_003225183.1 NZ_CP026010:1637959-1638240 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT92651 NZ_CP026010:1637959-1638240 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References