Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1469487..1470223 Replicon chromosome
Accession NZ_CP025963
Organism Klebsiella pneumoniae strain WCHKP020034

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag CEA73_RS07450 Protein ID WP_032433360.1
Coordinates 1469741..1470223 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CEA73_RS07445 Protein ID WP_003026799.1
Coordinates 1469487..1469753 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEA73_RS07420 1465133..1466272 + 1140 WP_032433368.1 mannitol dehydrogenase -
CEA73_RS07425 1466301..1466963 + 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
CEA73_RS07430 1466947..1467951 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
CEA73_RS07435 1467969..1468601 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
CEA73_RS07440 1468611..1469174 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
CEA73_RS07445 1469487..1469753 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CEA73_RS07450 1469741..1470223 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
CEA73_RS07455 1470584..1471183 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
CEA73_RS07460 1471396..1472340 - 945 WP_077254249.1 fimbrial protein -
CEA73_RS07465 1472352..1472930 - 579 WP_032432061.1 type 1 fimbrial protein -
CEA73_RS07470 1472934..1473674 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1437432..1477985 40553


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T92592 WP_032433360.1 NZ_CP025963:1469741-1470223 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T92592 NZ_CP025963:1469741-1470223 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT92592 WP_003026799.1 NZ_CP025963:1469487-1469753 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT92592 NZ_CP025963:1469487-1469753 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References