Detailed information of TA system TA092529

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32757..33026
• Replicon: plasmid pCTXM55_020023
• Accession: NZ_CP025949
• Organism: Escherichia coli strain SCEC020023

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C1A20_RS01220
• Protein ID: WP_001312861.1
• Coordinates: 32868..33026 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32757..32822 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1A20_RS01195	28467..28994	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
C1A20_RS01200	29052..29285	+	234	WP_000006003.1	DUF905 family protein	-
C1A20_RS01205	29346..31369	+	2024	Protein_39	ParB/RepB/Spo0J family partition protein	-
C1A20_RS01210	31438..31872	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C1A20_RS01215	31869..32588	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	32600..32824	+	225	NuclAT_0	-	-
-	32600..32824	+	225	NuclAT_0	-	-
-	32600..32824	+	225	NuclAT_0	-	-
-	32600..32824	+	225	NuclAT_0	-	-
C1A20_RS25235	32609..32788	-	180	WP_001309233.1	hypothetical protein	-
-	32757..32822	+	66	-	-	Antitoxin
C1A20_RS01220	32868..33026	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C1A20_RS25430	33264..33641	-	378	Protein_44	hypothetical protein	-
C1A20_RS01240	33941..34237	+	297	WP_001272251.1	hypothetical protein	-
C1A20_RS01245	34348..35169	+	822	WP_001234445.1	DUF945 domain-containing protein	-
C1A20_RS01250	35466..36113	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
C1A20_RS01255	36390..36773	+	384	WP_000124981.1	relaxosome protein TraM	-
C1A20_RS01260	36964..37650	+	687	WP_015059009.1	PAS domain-containing protein	-
C1A20_RS01265	37744..37971	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / blaTEM-1B / rmtB / blaCTX-M-55	-	1..73313	73313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T92529 WP_001312861.1 NZ_CP025949:32868-33026 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T92529 NZ_CP025949:32868-33026 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT92529 NZ_CP025949:32757-32822 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin