Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1586313..1586967 Replicon chromosome
Accession NZ_CP025851
Organism Escherichia coli strain 510016

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CXX11_RS07990 Protein ID WP_000244777.1
Coordinates 1586313..1586720 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CXX11_RS07995 Protein ID WP_000354046.1
Coordinates 1586701..1586967 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX11_RS07970 1582270..1584003 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
CXX11_RS07975 1584009..1584719 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CXX11_RS07980 1584744..1585640 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CXX11_RS07985 1585752..1586273 + 522 WP_001055874.1 flavodoxin FldB -
CXX11_RS07990 1586313..1586720 - 408 WP_000244777.1 toxin CptA Toxin
CXX11_RS07995 1586701..1586967 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CXX11_RS08000 1587210..1588190 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CXX11_RS08005 1588386..1589045 - 660 WP_047084947.1 hemolysin III family protein -
CXX11_RS08010 1589209..1589520 - 312 WP_001400966.1 N(4)-acetylcytidine aminohydrolase -
CXX11_RS08015 1589565..1590998 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
CXX11_RS08020 1591055..1591798 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T92125 WP_000244777.1 NZ_CP025851:c1586720-1586313 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T92125 NZ_CP025851:c1586720-1586313 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTTAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT92125 WP_000354046.1 NZ_CP025851:c1586967-1586701 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT92125 NZ_CP025851:c1586967-1586701 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References