Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 686513..687015 Replicon chromosome
Accession NZ_CP025851
Organism Escherichia coli strain 510016

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX11_RS03650 Protein ID WP_000767829.1
Coordinates 686513..686767 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX11_RS03655 Protein ID WP_001259255.1
Coordinates 686764..687015 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX11_RS03620 681528..681755 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CXX11_RS03625 681769..682827 - 1059 WP_000492340.1 transport protein YeeE -
CXX11_RS03630 683006..684364 - 1359 WP_097346625.1 putrescine/proton symporter PlaP -
CXX11_RS03635 684354..684416 - 63 WP_010723108.1 membrane protein YoeI -
CXX11_RS03640 684631..685560 - 930 WP_000803337.1 LysR family transcriptional regulator -
CXX11_RS03645 685606..686430 - 825 WP_000754737.1 SDR family oxidoreductase -
CXX11_RS03650 686513..686767 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX11_RS03655 686764..687015 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX11_RS03660 687298..687348 + 51 WP_001364200.1 his operon leader peptide -
CXX11_RS03665 687494..688393 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXX11_RS03670 688399..689697 + 1299 WP_001400564.1 histidinol dehydrogenase -
CXX11_RS03675 689700..690770 + 1071 WP_000108954.1 histidinol-phosphate transaminase -
CXX11_RS03680 690770..691837 + 1068 WP_001400565.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92123 WP_000767829.1 NZ_CP025851:c686767-686513 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92123 NZ_CP025851:c686767-686513 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92123 WP_001259255.1 NZ_CP025851:c687015-686764 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92123 NZ_CP025851:c687015-686764 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References