Detailed information of TA system TA092087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 89887..90412
• Replicon: plasmid p602354_148
• Accession: NZ_CP025848
• Organism: Escherichia coli strain 602354

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: T2HS38
• Locus tag: CXX15_RS25500
• Protein ID: WP_001159861.1
• Coordinates: 90107..90412 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: T2HUI2
• Locus tag: CXX15_RS25495
• Protein ID: WP_000813631.1
• Coordinates: 89887..90105 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX15_RS25460	85563..86681	-	1119	Protein_102	IS3 family transposase	-
CXX15_RS25465	86857..87273	-	417	WP_001044769.1	type II toxin-antitoxin system VapC family toxin	-
CXX15_RS25470	87270..87500	-	231	WP_001261281.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CXX15_RS25480	87992..88609	+	618	WP_001398801.1	RES family NAD+ phosphorylase	-
CXX15_RS25485	88643..89332	-	690	WP_001095858.1	helix-turn-helix domain-containing protein	-
CXX15_RS25495	89887..90105	+	219	WP_000813631.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CXX15_RS25500	90107..90412	+	306	WP_001159861.1	type II toxin-antitoxin system toxin CcdB	Toxin
CXX15_RS25505	90413..91219	+	807	WP_000099345.1	site-specific integrase	-
CXX15_RS25510	91576..91956	+	381	WP_001466366.1	IS66 family insertion sequence hypothetical protein	-
CXX15_RS25515	91953..92300	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
CXX15_RS25525	92522..93304	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
CXX15_RS25530	93301..94323	-	1023	WP_001356151.1	IS21 family transposase	-
CXX15_RS25535	94410..94787	+	378	Protein_114	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	csfA / cssA	1..148526	148526
	-	inside	IScluster/Tn	-	-	85654..103751	18097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11680.49 Da
• Isoelectric Point: 6.4674

>T92087 WP_001159861.1 NZ_CP025848:90107-90412 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINMMFWGI

Nucleotide

• Length: 306 bp

>T92087 NZ_CP025848:90107-90412 [Escherichia coli]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTGCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGACTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATTGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAATGCCATTAACATGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT92087 WP_000813631.1 NZ_CP025848:89887-90105 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMKNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT92087 NZ_CP025848:89887-90105 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTACTCAAAGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGAAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: T2HS38

Antitoxin

• AlphaFold DB: T2HUI2