Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4895285..4895787 Replicon chromosome
Accession NZ_CP025847
Organism Escherichia coli strain 602354

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX15_RS24105 Protein ID WP_000767829.1
Coordinates 4895285..4895539 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX15_RS24110 Protein ID WP_001259255.1
Coordinates 4895536..4895787 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX15_RS24075 4890300..4890527 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CXX15_RS24080 4890541..4891599 - 1059 WP_000492305.1 transport protein YeeE -
CXX15_RS24085 4891778..4893136 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX15_RS24090 4893126..4893188 - 63 WP_010723108.1 membrane protein YoeI -
CXX15_RS24095 4893403..4894332 - 930 WP_000803360.1 LysR family transcriptional regulator -
CXX15_RS24100 4894378..4895202 - 825 WP_000754737.1 SDR family oxidoreductase -
CXX15_RS24105 4895285..4895539 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX15_RS24110 4895536..4895787 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX15_RS24115 4896070..4896120 + 51 WP_001364200.1 his operon leader peptide -
CXX15_RS24120 4896266..4897165 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXX15_RS24125 4897171..4898475 + 1305 WP_001355813.1 histidinol dehydrogenase -
CXX15_RS24130 4898472..4899542 + 1071 WP_001099220.1 histidinol-phosphate transaminase -
CXX15_RS24135 4899542..4900609 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92085 WP_000767829.1 NZ_CP025847:c4895539-4895285 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92085 NZ_CP025847:c4895539-4895285 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92085 WP_001259255.1 NZ_CP025847:c4895787-4895536 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92085 NZ_CP025847:c4895787-4895536 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References