Detailed information of TA system TA091815

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127036..127457
• Replicon: plasmid pYDC107_184
• Accession: NZ_CP025708
• Organism: Escherichia coli strain YDC107

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: YDC107_RS26250
• Protein ID: WP_001302184.1
• Coordinates: 127036..127194 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	127259..127457 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YDC107_RS26215	122100..122315	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
YDC107_RS26220	122451..123098	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
YDC107_RS26225	123289..123672	-	384	WP_001063020.1	relaxosome protein TraM	-
YDC107_RS26230	124012..124614	+	603	WP_077634248.1	transglycosylase SLT domain-containing protein	-
YDC107_RS26235	124910..125731	-	822	WP_001234465.1	DUF945 domain-containing protein	-
YDC107_RS26240	125850..126137	-	288	WP_000107242.1	hypothetical protein	-
YDC107_RS26245	126201..126798	+	598	Protein_176	hypothetical protein	-
YDC107_RS26250	127036..127194	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	127259..127457	-	199	NuclAT_0	-	Antitoxin
-	127259..127457	-	199	NuclAT_0	-	Antitoxin
-	127259..127457	-	199	NuclAT_0	-	Antitoxin
-	127259..127457	-	199	NuclAT_0	-	Antitoxin
YDC107_RS26255	127426..127531	-	106	Protein_178	plasmid SOS inhibition protein A	-
YDC107_RS26260	127686..128003	+	318	Protein_179	IS110 family transposase	-
YDC107_RS26265	128292..128543	-	252	Protein_180	iron-containing alcohol dehydrogenase	-
YDC107_RS26270	128628..129251	-	624	WP_000912556.1	potassium channel family protein	-
YDC107_RS26275	129267..129960	-	694	Protein_182	IS1 family transposase	-
YDC107_RS26280	130017..130121	+	105	Protein_183	thiosulfate reductase cytochrome B subunit	-
YDC107_RS26285	130188..130367	-	180	WP_001553840.1	hypothetical protein	-
YDC107_RS26290	130367..131761	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14	-	1..184098	184098
	-	flank	IS/Tn	-	-	129267..129644	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T91815 WP_001302184.1 NZ_CP025708:c127194-127036 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T91815 NZ_CP025708:c127194-127036 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT91815 NZ_CP025708:c127457-127259 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin