Detailed information of TA system TA091813

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89610..89864
• Replicon: plasmid pYDC107_184
• Accession: NZ_CP025708
• Organism: Escherichia coli strain YDC107

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: YDC107_RS26015
• Protein ID: WP_001312851.1
• Coordinates: 89610..89759 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	89803..89864 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YDC107_RS25980	84857..85768	-	912	WP_000440183.1	carbamate kinase	-
YDC107_RS25985	85779..86999	-	1221	WP_000410951.1	arginine deiminase	-
YDC107_RS25990	87892..88374	+	483	Protein_125	hypothetical protein	-
YDC107_RS25995	88320..88766	-	447	Protein_126	incFII family plasmid replication initiator RepA	-
YDC107_RS26000	88759..88833	-	75	WP_001365705.1	RepA leader peptide Tap	-
YDC107_RS26005	88917..89033	+	117	WP_071586949.1	replication protein RepA	-
YDC107_RS26010	89069..89326	-	258	WP_000083833.1	replication regulatory protein RepA	-
YDC107_RS26015	89610..89759	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89803..89864	+	62	NuclAT_1	-	Antitoxin
-	89803..89864	+	62	NuclAT_1	-	Antitoxin
-	89803..89864	+	62	NuclAT_1	-	Antitoxin
-	89803..89864	+	62	NuclAT_1	-	Antitoxin
YDC107_RS26020	89815..90057	+	243	WP_023149666.1	hypothetical protein	-
YDC107_RS26025	90003..90236	-	234	WP_072142979.1	hypothetical protein	-
YDC107_RS26030	90286..90988	+	703	Protein_133	IS1 family transposase	-
YDC107_RS26035	90982..91104	-	123	WP_001496308.1	hypothetical protein	-
YDC107_RS26040	91289..91501	-	213	WP_005012601.1	hypothetical protein	-
YDC107_RS26045	91634..92194	-	561	WP_000139363.1	fertility inhibition protein FinO	-
YDC107_RS26050	92249..92995	-	747	WP_000205701.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14	-	1..184098	184098
	-	flank	IS/Tn	-	-	90602..90988	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T91813 WP_001312851.1 NZ_CP025708:c89759-89610 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T91813 NZ_CP025708:c89759-89610 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT91813 NZ_CP025708:89803-89864 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin