Detailed information of TA system TA090599

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 121913..122151
• Replicon: plasmid pE1246_1
• Accession: NZ_CP025574
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: CY655_RS27670
• Protein ID: WP_023144756.1
• Coordinates: 122017..122151 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	121913..121964 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS27645	119443..120189	+	747	WP_000205736.1	conjugal transfer pilus acetylase TraX	-
CY655_RS27650	120244..120804	+	561	WP_000139341.1	fertility inhibition protein FinO	-
CY655_RS27655	120936..121148	+	213	WP_001301409.1	ANR family transcriptional regulator	-
CY655_RS28010	121660..121946	+	287	Protein_144	DUF2726 domain-containing protein	-
-	121913..121964	-	52	NuclAT_1	-	Antitoxin
-	121913..121964	-	52	NuclAT_1	-	Antitoxin
-	121913..121964	-	52	NuclAT_1	-	Antitoxin
-	121913..121964	-	52	NuclAT_1	-	Antitoxin
CY655_RS27670	122017..122151	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
CY655_RS27675	122424..122678	+	255	WP_000083850.1	replication regulatory protein RepA	-
CY655_RS28015	122784..122918	+	135	Protein_147	protein CopA/IncA	-
CY655_RS27685	122915..122989	+	75	WP_031943482.1	RepA leader peptide Tap	-
CY655_RS27690	122982..123839	+	858	WP_032284166.1	incFII family plasmid replication initiator RepA	-
CY655_RS27705	124757..125026	+	270	WP_000079941.1	hypothetical protein	-
CY655_RS27710	125023..125304	+	282	WP_001402901.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CY655_RS27715	125350..126189	+	840	WP_032269705.1	3'-5' exonuclease	-
CY655_RS27720	126306..126788	+	483	WP_001402899.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	agg3D / agg3C / agg3B / aap/aspU / pic	1..129195	129195
	-	flank	IS/Tn	-	-	126948..127970	1022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T90599 WP_023144756.1 NZ_CP025574:122017-122151 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T90599 NZ_CP025574:122017-122151 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 52 bp

>AT90599 NZ_CP025574:c121964-121913 [Escherichia coli]
TCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin