Detailed information of TA system TA090547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 437711..438343
• Replicon: chromosome
• Accession: NZ_CP025545
• Organism: Brevibacillus laterosporus strain ZQ2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7EEZ8
• Locus tag: C0R09_RS02220
• Protein ID: WP_003333534.1
• Coordinates: 437993..438343 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: C0R09_RS02215
• Protein ID: WP_173645602.1
• Coordinates: 437711..437989 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0R09_RS02190	433446..433778	+	333	WP_003333541.1	metalloregulator ArsR/SmtB family transcription factor	-
C0R09_RS02195	433989..434312	+	324	WP_101667303.1	gamma-type small acid-soluble spore protein	-
C0R09_RS02200	434634..435665	+	1032	WP_003333538.1	DUF4367 domain-containing protein	-
C0R09_RS02205	435893..436336	+	444	WP_003333537.1	hypothetical protein	-
C0R09_RS02210	436340..437545	+	1206	WP_101667304.1	alanine racemase	-
C0R09_RS02215	437711..437989	+	279	WP_173645602.1	ribbon-helix-helix protein, CopG family	Antitoxin
C0R09_RS02220	437993..438343	+	351	WP_003333534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C0R09_RS02225	438573..440732	+	2160	WP_101667305.1	RNA-binding transcriptional accessory protein	-
C0R09_RS02230	440804..440929	-	126	WP_003333532.1	cortex morphogenetic protein CmpA	-
C0R09_RS02235	441041..441376	-	336	WP_031411755.1	hypothetical protein	-
C0R09_RS02240	441495..441959	+	465	WP_003333530.1	SprT family protein	-
C0R09_RS02245	442025..442495	+	471	WP_064019190.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
C0R09_RS02250	442476..443189	+	714	WP_022586371.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.83 Da
• Isoelectric Point: 4.8553

>T90547 WP_003333534.1 NZ_CP025545:437993-438343 [Brevibacillus laterosporus]
VNVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T90547 NZ_CP025545:437993-438343 [Brevibacillus laterosporus]
GTGAATGTTAAACGTGGCGATGTATTTTTCGCGGACCTTTCCCCTGTGGTTGGCTCCGAGCAAGGTGGAGTTAGACCGGT
ATTAGTTATACAAAACGATATTGGAAATCGATTTAGTCCGACGGTTATCGTCGCTGCGATCACTGCTCAGATTCAAAAGG
CAAAGCTGCCTACGCATGTGGAAATCGATGCAAAAACATATGGGTTTGATCGTGACTCTGTCATCTTATTGGAACAAATA
AGAACCATTGATAAGCAAAGGTTGACTGATAAAATCACTCATCTAGACGATGAGATGATGGAAAGAGTGAATGAATCACT
TCAGATCAGCCTCGGATTAATAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.43 Da
• Isoelectric Point: 9.6543

>AT90547 WP_173645602.1 NZ_CP025545:437711-437989 [Brevibacillus laterosporus]
MSKSNTKRIMISLPQHLLQEVDGVVQKERSNRSELIRQAMSLYLKERKKRFIRETMQRGYLEMAKINLNMASEAFEAEEE
ADLTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT90547 NZ_CP025545:437711-437989 [Brevibacillus laterosporus]
TTGTCTAAAAGCAATACAAAACGTATCATGATCAGTTTGCCGCAACATTTATTGCAAGAGGTAGACGGCGTCGTGCAGAA
AGAGAGATCAAACCGAAGTGAATTGATTCGTCAAGCGATGAGTCTCTACCTCAAGGAACGAAAAAAACGCTTTATACGAG
AAACAATGCAACGTGGCTATTTGGAAATGGCTAAAATCAATCTCAATATGGCATCGGAGGCTTTTGAAGCAGAAGAGGAA
GCCGATCTTACCTTAGACCGCTTAGTTAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A0F7EEZ8