Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1324863..1325427 Replicon chromosome
Accession NZ_CP025499
Organism Lacticaseibacillus paracasei subsp. tolerans strain AO356

Toxin (Protein)


Gene name yafQ Uniprot ID A0A6G9EML4
Locus tag CYL78_RS07510 Protein ID WP_014951859.1
Coordinates 1325125..1325427 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag CYL78_RS07505 Protein ID WP_003570051.1
Coordinates 1324863..1325132 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYL78_RS07475 1319867..1320754 - 888 WP_024109452.1 ABC transporter ATP-binding protein -
CYL78_RS07480 1320952..1322178 - 1227 WP_012491507.1 hypothetical protein -
CYL78_RS07485 1322376..1322780 + 405 WP_012491508.1 MerR family transcriptional regulator -
CYL78_RS07490 1322773..1323099 + 327 WP_012491509.1 YrdB family protein -
CYL78_RS07495 1323162..1323791 + 630 WP_012491510.1 DUF2399 domain-containing protein -
CYL78_RS07500 1323836..1324489 + 654 WP_012491511.1 GNAT family N-acetyltransferase -
CYL78_RS07505 1324863..1325132 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CYL78_RS07510 1325125..1325427 + 303 WP_014951859.1 type II toxin-antitoxin system YafQ family toxin Toxin
CYL78_RS16340 1325766..1325909 + 144 WP_003574774.1 hypothetical protein -
CYL78_RS07515 1326027..1326719 + 693 WP_012491513.1 hypothetical protein -
CYL78_RS07520 1326955..1329132 + 2178 WP_012491514.1 RNA-binding transcriptional accessory protein -
CYL78_RS07525 1329197..1330126 - 930 WP_003599410.1 IS30-like element ISLpl1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1329197..1330126 929


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-98)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11441.27 Da        Isoelectric Point: 10.2818

>T90450 WP_014951859.1 NZ_CP025499:1325125-1325427 [Lacticaseibacillus paracasei subsp. tolerans]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELHVDGPRGDWLLIYKIK
QQDLILTLVRTGSHHNLLGK

Download         Length: 303 bp

>T90450 NZ_CP025499:1325125-1325427 [Lacticaseibacillus paracasei subsp. tolerans]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCTGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCTT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTACATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTAAG
CAGCAAGATCTCATTTTGACCCTGGTTAGAACTGGATCTCATCATAACCTTTTGGGTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT90450 WP_003570051.1 NZ_CP025499:1324863-1325132 [Lacticaseibacillus paracasei subsp. tolerans]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT90450 NZ_CP025499:1324863-1325132 [Lacticaseibacillus paracasei subsp. tolerans]
ATGGCAGCCACAAAGAAAGAAACTCGCTTGAATATTCGTGTTGATCCGGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACTATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G9EML4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References