90384

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 141182..141704 Replicon plasmid p44-1
Accession NZ_CP025462
Organism Klebsiella pneumoniae strain F44

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag CYE02_RS28610 Protein ID WP_004181778.1
Coordinates 141182..141466 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag CYE02_RS28615 Protein ID WP_004181777.1
Coordinates 141456..141704 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CYE02_RS28580 136383..137478 + 1096 Protein_134 conjugal transfer protein TraG N-terminal domain-containing protein -
CYE02_RS28585 137577..138764 - 1188 WP_040209642.1 transposase -
CYE02_RS28590 138800..139228 + 429 WP_077257669.1 IS200/IS605 family transposase -
CYE02_RS28595 139307..139531 + 225 Protein_137 transposase -
CYE02_RS28600 139802..141028 - 1227 WP_040209639.1 transposase -
CYE02_RS28605 141064..141165 + 102 Protein_139 IS200/IS605 family transposase -
CYE02_RS28610 141182..141466 - 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CYE02_RS28615 141456..141704 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CYE02_RS28620 141995..143794 + 1800 WP_004181776.1 ATP-dependent helicase -
CYE02_RS28625 144128..145114 + 987 WP_101992392.1 hypothetical protein -
CYE02_RS28630 145259..145612 + 354 WP_004181774.1 hypothetical protein -
CYE02_RS28635 145674..146495 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / blaTEM-1B / aac(3)-IId / blaSHV-12 rmpA / iutA / iucD / iucC / iucB / iucA 1..261706 261706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T90384 WP_004181778.1 NZ_CP025462:c141466-141182 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T90384 NZ_CP025462:c141466-141182 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT90384 WP_004181777.1 NZ_CP025462:c141704-141456 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT90384 NZ_CP025462:c141704-141456 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References