Detailed information of TA system TA090128

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 106277..106702
• Replicon: plasmid unnamed
• Accession: NZ_CP025329
• Organism: Escherichia coli strain ExPEC XM

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: CXG97_RS27325
• Protein ID: WP_001332444.1
• Coordinates: 106541..106702 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	106277..106488 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXG97_RS27295	102251..102748	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
CXG97_RS27300	102811..103044	+	234	WP_001209608.1	DUF905 domain-containing protein	-
CXG97_RS27305	103103..105061	+	1959	Protein_91	ParB/RepB/Spo0J family partition protein	-
CXG97_RS27310	105116..105550	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
CXG97_RS27315	105547..106266	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	106277..106488	+	212	NuclAT_1	-	Antitoxin
-	106277..106488	+	212	NuclAT_1	-	Antitoxin
-	106277..106488	+	212	NuclAT_1	-	Antitoxin
-	106277..106488	+	212	NuclAT_1	-	Antitoxin
CXG97_RS27325	106541..106702	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
CXG97_RS27330	106940..107552	-	613	Protein_95	hypothetical protein	-
CXG97_RS27335	107731..108552	+	822	WP_001234485.1	DUF945 domain-containing protein	-
CXG97_RS27340	108848..109438	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
CXG97_RS27350	109772..110155	+	384	WP_000124981.1	relaxosome protein TraM	-
CXG97_RS27355	110345..111031	+	687	WP_000332490.1	PAS domain-containing protein	-
CXG97_RS27360	111125..111352	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(6)-Id / aph(3'')-Ib / tet(A)	vat / iroN / iroE / iroD / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..286854	286854
	-	flank	IS/Tn	-	-	100902..101405	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T90128 WP_001332444.1 NZ_CP025329:106541-106702 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T90128 NZ_CP025329:106541-106702 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT90128 NZ_CP025329:106277-106488 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin