Detailed information of TA system TA090062

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2012620..2012845
• Replicon: chromosome
• Accession: NZ_CP025317
• Organism: Escherichia albertii strain 1551-2

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A5D1NM67
• Locus tag: CXP54_RS10440
• Protein ID: WP_000813262.1
• Coordinates: 2012620..2012775 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2012787..2012845 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXP54_RS10405	2008302..2009351	-	1050	WP_032189571.1	site-specific DNA-methyltransferase	-
CXP54_RS10410	2009502..2009699	-	198	WP_000917767.1	hypothetical protein	-
CXP54_RS10415	2009927..2010748	-	822	WP_000762914.1	antitermination protein	-
CXP54_RS10420	2010745..2011119	-	375	WP_001217453.1	RusA family crossover junction endodeoxyribonuclease	-
CXP54_RS10425	2011132..2012178	-	1047	WP_001265093.1	DUF968 domain-containing protein	-
CXP54_RS10430	2012180..2012452	-	273	WP_001329966.1	hypothetical protein	-
CXP54_RS10440	2012620..2012775	-	156	WP_000813262.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2012787..2012845	+	59	-	-	Antitoxin
CXP54_RS10445	2013013..2013678	-	666	WP_071290413.1	epoxyqueuosine reductase QueH	-
CXP54_RS10450	2013853..2014278	-	426	WP_021546098.1	DUF977 family protein	-
CXP54_RS10455	2014319..2015389	-	1071	WP_023154928.1	phage replisome organizer protein	-
CXP54_RS10460	2015461..2015886	-	426	WP_000693835.1	Rha family transcriptional regulator	-
CXP54_RS10465	2015870..2016151	-	282	WP_000391950.1	helix-turn-helix domain-containing protein	-
CXP54_RS10470	2016252..2016671	+	420	WP_000362155.1	hypothetical protein	-
CXP54_RS10475	2016937..2017092	+	156	WP_000379575.1	DUF1391 family protein	-
CXP54_RS10485	2017252..2017470	+	219	WP_001171942.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleH1 / nleC / nleB2	1976045..2045541	69496

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T90062 WP_000813262.1 NZ_CP025317:c2012775-2012620 [Escherichia albertii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRLRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T90062 NZ_CP025317:c2012775-2012620 [Escherichia albertii]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGACTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT90062 NZ_CP025317:2012787-2012845 [Escherichia albertii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A5D1NM67

Antitoxin