Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3042720..3043374 Replicon chromosome
Accession NZ_CP025268
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag CXP41_RS15835 Protein ID WP_000244777.1
Coordinates 3042720..3043127 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag CXP41_RS15840 Protein ID WP_000354046.1
Coordinates 3043108..3043374 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXP41_RS15815 3038677..3040410 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
CXP41_RS15820 3040416..3041126 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
CXP41_RS15825 3041151..3042047 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
CXP41_RS15830 3042159..3042680 + 522 WP_001055874.1 flavodoxin FldB -
CXP41_RS15835 3042720..3043127 - 408 WP_000244777.1 toxin CptA Toxin
CXP41_RS15840 3043108..3043374 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
CXP41_RS15845 3043617..3044597 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
CXP41_RS15850 3044793..3045452 - 660 WP_000250274.1 hemolysin III family protein -
CXP41_RS15855 3045616..3045927 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
CXP41_RS15860 3045972..3047405 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
CXP41_RS15865 3047462..3048205 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T90010 WP_000244777.1 NZ_CP025268:c3043127-3042720 [Escherichia coli str. K-12 substr. MG1655]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T90010 NZ_CP025268:c3043127-3042720 [Escherichia coli str. K-12 substr. MG1655]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT90010 WP_000354046.1 NZ_CP025268:c3043374-3043108 [Escherichia coli str. K-12 substr. MG1655]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT90010 NZ_CP025268:c3043374-3043108 [Escherichia coli str. K-12 substr. MG1655]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References