Detailed information of TA system TA089933

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 378949..379784
• Replicon: chromosome
• Accession: NZ_CP025251
• Organism: Escherichia coli strain BH100 substr. MG2017

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q83W73
• Locus tag: BH100B_RS01890
• Protein ID: WP_000854700.1
• Coordinates: 379407..379784 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E3PJ72
• Locus tag: BH100B_RS01885
• Protein ID: WP_001278232.1
• Coordinates: 378949..379317 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100B_RS01865	376609..377427	+	819	WP_001234709.1	DUF945 domain-containing protein	-
BH100B_RS01870	377519..378004	+	486	WP_000213723.1	antirestriction protein	-
BH100B_RS01875	378020..378496	+	477	WP_001186199.1	RadC family protein	-
BH100B_RS01880	378565..378786	+	222	WP_000692353.1	DUF987 domain-containing protein	-
BH100B_RS01885	378949..379317	+	369	WP_001278232.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
BH100B_RS01890	379407..379784	+	378	WP_000854700.1	TA system toxin CbtA family protein	Toxin
BH100B_RS01895	379781..380269	+	489	WP_000761726.1	hypothetical protein	-
BH100B_RS01900	380281..380478	+	198	WP_000839247.1	DUF957 domain-containing protein	-
BH100B_RS01905	380563..381417	+	855	WP_024174313.1	DUF4942 domain-containing protein	-
BH100B_RS01910	381510..382829	-	1320	WP_000144686.1	site-specific integrase	-
BH100B_RS01915	383195..383533	+	339	WP_000772639.1	Arm DNA-binding domain-containing protein	-
BH100B_RS01920	383968..384462	-	495	WP_001059463.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	vat / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	353224..428763	75539

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14276.28 Da
• Isoelectric Point: 7.8046

>T89933 WP_000854700.1 NZ_CP025251:379407-379784 [Escherichia coli]
MKTLPDTHVREASRCLSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SPCTRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Nucleotide

• Length: 378 bp

>T89933 NZ_CP025251:379407-379784 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13491.37 Da
• Isoelectric Point: 6.6290

>AT89933 WP_001278232.1 NZ_CP025251:378949-379317 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89933 NZ_CP025251:378949-379317 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A454A1I2

Antitoxin

• AlphaFold DB: E3PJ72