Detailed information of TA system TA089893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 71629..72154
• Replicon: plasmid unnamed2
• Accession: NZ_CP025213
• Organism: Klebsiella pneumoniae strain HZW25

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: CW753_RS27455
• Protein ID: WP_016946795.1
• Coordinates: 71849..72154 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: CW753_RS27450
• Protein ID: WP_004197642.1
• Coordinates: 71629..71847 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CW753_RS27415 (CW753_27605)	67091..67441	+	351	WP_167878626.1	hypothetical protein	-
CW753_RS30205	67541..67663	+	123	WP_256046161.1	hypothetical protein	-
CW753_RS27425 (CW753_27615)	68082..70220	+	2139	WP_048295729.1	AAA family ATPase	-
CW753_RS27430 (CW753_27620)	70390..70806	-	417	WP_048295728.1	type II toxin-antitoxin system VapC family toxin	-
CW753_RS27435 (CW753_27625)	70803..71033	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	-
CW753_RS27450 (CW753_27640)	71629..71847	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CW753_RS27455 (CW753_27645)	71849..72154	+	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
CW753_RS27460 (CW753_27650)	72312..72818	+	507	WP_023294776.1	hypothetical protein	-
CW753_RS27465 (CW753_27655)	72997..73773	+	777	WP_049245386.1	site-specific integrase	-
CW753_RS27470 (CW753_27660)	73831..74088	-	258	WP_000764642.1	hypothetical protein	-
CW753_RS29680	74217..74321	-	105	WP_032409716.1	hypothetical protein	-
CW753_RS27475 (CW753_27665)	74855..75721	+	867	WP_004118283.1	replication initiation protein	-
CW753_RS27480 (CW753_27670)	76077..76346	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..103957	103957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T89893 WP_016946795.1 NZ_CP025213:71849-72154 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T89893 NZ_CP025213:71849-72154 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT89893 WP_004197642.1 NZ_CP025213:71629-71847 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT89893 NZ_CP025213:71629-71847 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA