Detailed information of TA system TA089774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3311300..3311862
• Replicon: chromosome
• Accession: NZ_CP025085
• Organism: Serratia sp. ATCC 39006 strain LacA

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I5T8X6
• Locus tag: CWC46_RS15105
• Protein ID: WP_021015725.1
• Coordinates: 3311300..3311578 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CWC46_RS15110
• Protein ID: WP_021015724.1
• Coordinates: 3311578..3311862 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWC46_RS15065	3306927..3307307	-	381	Protein_2910	transposase	-
CWC46_RS15070	3307456..3307746	+	291	WP_021015729.1	hypothetical protein	-
CWC46_RS15075	3307897..3308298	+	402	WP_021015728.1	nuclear transport factor 2 family protein	-
CWC46_RS15080	3308675..3309127	-	453	WP_021015727.1	hypothetical protein	-
CWC46_RS15090	3310132..3310479	+	348	WP_021015726.1	hypothetical protein	-
CWC46_RS15095	3310778..3310957	-	180	WP_037380902.1	hypothetical protein	-
CWC46_RS15105	3311300..3311578	+	279	WP_021015725.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWC46_RS15110	3311578..3311862	+	285	WP_021015724.1	HigA family addiction module antidote protein	Antitoxin
CWC46_RS15115	3312093..3312428	+	336	WP_084297613.1	tyrosine-type recombinase/integrase	-
CWC46_RS15120	3312743..3313081	-	339	WP_037381545.1	YebY family protein	-
CWC46_RS15125	3313315..3313824	-	510	WP_021015721.1	non-heme ferritin	-
CWC46_RS15130	3314348..3314578	+	231	WP_021015713.1	DNA polymerase III subunit theta	-
CWC46_RS15135	3314626..3315291	-	666	WP_157831397.1	copper homeostasis membrane protein CopD	-
CWC46_RS15140	3315330..3316589	-	1260	WP_021015719.1	ATP-grasp domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10487.93 Da
• Isoelectric Point: 9.4011

>T89774 WP_021015725.1 NZ_CP025085:3311300-3311578 [Serratia sp. ATCC 39006]
MIKSFKHKGLRQFFEKGSTAGIDAKQAGKIRLRLAVIDAAEEIKDIDRPGYSLHPLTGDKKGQWSITVTGNWRITFEFID
GNAYIVNYEDYH

Nucleotide

• Length: 279 bp

>T89774 NZ_CP025085:3311300-3311578 [Serratia sp. ATCC 39006]
ATGATTAAAAGCTTCAAGCATAAAGGGTTGCGTCAGTTCTTCGAAAAAGGCTCCACCGCAGGAATAGACGCAAAACAAGC
TGGGAAAATCAGGCTTCGTCTCGCGGTTATTGATGCAGCGGAAGAGATAAAGGATATTGACCGCCCTGGCTACAGTCTCC
ACCCGTTAACCGGAGACAAAAAAGGGCAATGGTCCATAACCGTCACAGGGAACTGGCGCATTACATTTGAATTTATCGAT
GGGAATGCCTACATCGTTAATTATGAGGATTACCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9945.49 Da
• Isoelectric Point: 6.7208

>AT89774 WP_021015724.1 NZ_CP025085:3311578-3311862 [Serratia sp. ATCC 39006]
MTMHNPPHPGEIIAETLEELGVGIRELARALGVAPSTVQRLVAGNAAISPEMAVKLSAVLGSSAKFWLNLQGNYSLWVAQ
QTVDTSTLHKLVAA

Nucleotide

• Length: 285 bp

>AT89774 NZ_CP025085:3311578-3311862 [Serratia sp. ATCC 39006]
ATGACTATGCATAATCCCCCGCATCCGGGGGAAATCATTGCCGAAACCCTAGAAGAATTGGGTGTAGGCATCAGAGAACT
AGCCAGGGCACTCGGTGTAGCGCCTTCCACTGTACAACGTCTTGTTGCAGGGAATGCTGCTATCAGTCCTGAAATGGCAG
TAAAACTATCTGCCGTTCTGGGTAGTTCGGCGAAATTCTGGCTAAACCTTCAGGGCAATTACAGTCTATGGGTAGCTCAG
CAGACCGTTGACACGTCAACCTTGCACAAACTTGTTGCTGCATGA

Structures

Toxin

• AlphaFold DB: A0A2I5T8X6