Detailed information of TA system TA089716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2227282..2227876
• Replicon: chromosome
• Accession: NZ_CP025072
• Organism: Bordetella parapertussis strain B160

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: K0M8J0
• Locus tag: CWR60_RS10615
• Protein ID: WP_010928352.1
• Coordinates: 2227694..2227876 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: K0MFV7
• Locus tag: CWR60_RS10610
• Protein ID: WP_010928351.1
• Coordinates: 2227282..2227671 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWR60_RS10585	2223125..2224165	+	1041	WP_003809635.1	phosphate ABC transporter substrate-binding protein PstS	-
CWR60_RS10590	2224300..2225316	+	1017	WP_010928350.1	phosphate ABC transporter permease subunit PstC	-
CWR60_RS10595	2225338..2226195	+	858	WP_003809638.1	phosphate ABC transporter permease PstA	-
CWR60_RS10600	2226240..2227016	+	777	WP_033448479.1	phosphate ABC transporter ATP-binding protein PstB	-
CWR60_RS10610	2227282..2227671	-	390	WP_010928351.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
CWR60_RS10615	2227694..2227876	-	183	WP_010928352.1	type II toxin-antitoxin system HicA family toxin	Toxin
CWR60_RS10620	2228284..2228982	+	699	WP_003809653.1	gluconate 2-dehydrogenase subunit 3 family protein	-
CWR60_RS10625	2228986..2230755	+	1770	WP_010928353.1	GMC family oxidoreductase	-
CWR60_RS10630	2230766..2232121	+	1356	WP_003809657.1	cytochrome c	-
CWR60_RS10635	2232133..2232873	-	741	WP_003809659.1	carbonic anhydrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-122)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6750.76 Da
• Isoelectric Point: 11.4018

>T89716 WP_010928352.1 NZ_CP025072:c2227876-2227694 [Bordetella parapertussis]
MNSTDLIQQPKADGWYRVHTVGSHHQYKHPTKPGKVTVPHPRKGLPTATQRSILKQAGLR

Nucleotide

• Length: 183 bp

>T89716 NZ_CP025072:c2227876-2227694 [Bordetella parapertussis]
ATGAACAGCACAGACCTGATCCAGCAACCGAAGGCCGACGGCTGGTATCGCGTGCATACCGTGGGTTCACACCACCAGTA
CAAGCACCCGACCAAGCCCGGCAAGGTCACCGTGCCGCATCCCAGGAAGGGCCTGCCGACAGCGACACAGCGCAGCATCC
TTAAGCAAGCCGGCCTGCGATAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14270.92 Da
• Isoelectric Point: 4.3014

>AT89716 WP_010928351.1 NZ_CP025072:c2227671-2227282 [Bordetella parapertussis]
MLYPIHVSKDEGSAYGAAFPDFPGCFAAADELQDLPRAAQEAVEAHFYGETERIPAPSAPEAWANDEDYQGGYWMMVDID
LSKVNTKAVRLNISLPENLVHRIDEEAKARRLSRSAFLAMAAEHEMADA

Nucleotide

• Length: 390 bp

>AT89716 NZ_CP025072:c2227671-2227282 [Bordetella parapertussis]
ATGCTCTACCCGATCCATGTTTCCAAAGACGAAGGCAGCGCCTACGGGGCCGCCTTCCCTGATTTTCCCGGCTGCTTTGC
AGCCGCCGATGAACTCCAGGACCTCCCCCGCGCGGCGCAGGAAGCCGTTGAGGCGCACTTCTACGGAGAGACCGAGCGTA
TCCCCGCCCCGTCCGCCCCTGAAGCGTGGGCCAACGATGAGGACTATCAAGGCGGCTATTGGATGATGGTCGATATCGAC
CTGTCGAAGGTCAACACGAAGGCCGTGCGGCTCAACATCAGCCTGCCGGAAAACCTGGTTCACCGTATCGACGAGGAAGC
CAAGGCACGACGCCTTTCGCGCTCAGCGTTTCTGGCCATGGCTGCGGAGCACGAAATGGCCGACGCCTAG

Structures

Toxin

• AlphaFold DB: K0M8J0

Antitoxin

• AlphaFold DB: K0MFV7