Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 627553..628167 Replicon chromosome
Accession NZ_CP025056
Organism Pseudomonas aeruginosa strain PB367

Toxin (Protein)


Gene name hicA Uniprot ID A6VBH9
Locus tag CWI20_RS02925 Protein ID WP_071534354.1
Coordinates 627553..627735 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A140SDX7
Locus tag CWI20_RS02930 Protein ID WP_012077229.1
Coordinates 627763..628167 (+) Length 135 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWI20_RS02905 624524..624667 + 144 WP_003085001.1 hypothetical protein -
CWI20_RS02910 624993..625328 - 336 WP_003085003.1 NERD domain-containing protein -
CWI20_RS02915 625421..626542 - 1122 WP_003085005.1 Fic family protein -
CWI20_RS02920 626801..626937 - 137 Protein_582 integrase -
CWI20_RS02925 627553..627735 + 183 WP_071534354.1 type II toxin-antitoxin system HicA family toxin Toxin
CWI20_RS02930 627763..628167 + 405 WP_012077229.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
CWI20_RS02935 628210..629181 - 972 WP_012077228.1 hypothetical protein -
CWI20_RS02940 629166..629864 - 699 WP_033896049.1 hypothetical protein -
CWI20_RS02945 629864..630403 - 540 WP_012074127.1 hypothetical protein -
CWI20_RS02950 630400..631320 - 921 WP_012074128.1 hypothetical protein -
CWI20_RS02955 631317..632027 - 711 WP_012074129.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 625421..678893 53472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(3-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-127)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6702.74 Da        Isoelectric Point: 10.4826

>T89684 WP_071534354.1 NZ_CP025056:627553-627735 [Pseudomonas aeruginosa]
MRSREVIDLLLEDGWYEVAVKGSHHQFKHPSKPGKVTVQHPSSTIPKGTLNNILKQAGLK

Download         Length: 183 bp

>T89684 NZ_CP025056:627553-627735 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin


Download         Length: 135 a.a.        Molecular weight: 14670.54 Da        Isoelectric Point: 4.4315

>AT89684 WP_012077229.1 NZ_CP025056:627763-628167 [Pseudomonas aeruginosa]
MKFPVVLHKDPDSDYGVTVPDVPGCFSAGATVSEALANVEEALALHFEGLVTDGEELPQPQDVDAHMKNPDFEGGVWAVV
DFDVTPYLGKAVRFNATLPEHLLQRIDERVKVDKRYQSRSGFLATAAMRELSVA

Download         Length: 405 bp

>AT89684 NZ_CP025056:627763-628167 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6VBH9

Antitoxin

Source ID Structure
AlphaFold DB A0A140SDX7

References