Detailed information of TA system TA089668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 9159..9709
• Replicon: plasmid pPB353_1
• Accession: NZ_CP025052
• Organism: Pseudomonas aeruginosa strain PB353

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CWI23_RS30875
• Protein ID: WP_019727125.1
• Coordinates: 9159..9434 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6B1YL09
• Locus tag: CWI23_RS30880
• Protein ID: WP_019727126.1
• Coordinates: 9434..9709 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI23_RS31575	4475..5056	+	582	WP_177281055.1	hypothetical protein	-
CWI23_RS30835	5063..5317	+	255	WP_074202351.1	hypothetical protein	-
CWI23_RS30840	5344..5580	+	237	WP_031757127.1	hypothetical protein	-
CWI23_RS30845	5891..6568	+	678	WP_034018017.1	SOS response-associated peptidase	-
CWI23_RS30850	6677..6979	+	303	WP_023131919.1	anti-CRISPR protein AcrE1	-
CWI23_RS30855	7002..7433	+	432	WP_100882994.1	anti-CRISPR protein AcrF3	-
CWI23_RS30860	7430..7669	+	240	WP_100882995.1	anti-CRISPR protein	-
CWI23_RS31355	7965..8285	+	321	WP_157814495.1	hypothetical protein	-
CWI23_RS30870	8652..9068	+	417	WP_033992331.1	hypothetical protein	-
CWI23_RS30875	9159..9434	-	276	WP_019727125.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CWI23_RS30880	9434..9709	-	276	WP_019727126.1	ribbon-helix-helix domain-containing protein	Antitoxin
CWI23_RS30885	9984..10370	+	387	WP_023123731.1	hypothetical protein	-
CWI23_RS30890	10431..10724	+	294	WP_019727128.1	hypothetical protein	-
CWI23_RS30900	11048..11356	+	309	WP_034055800.1	hypothetical protein	-
CWI23_RS30905	11349..11681	+	333	WP_034055802.1	hypothetical protein	-
CWI23_RS30915	11975..12223	+	249	WP_058130600.1	hypothetical protein	-
CWI23_RS30920	12273..12524	-	252	WP_058130599.1	hypothetical protein	-
CWI23_RS30925	12583..12867	+	285	WP_019727136.1	hypothetical protein	-
CWI23_RS30930	12910..13287	+	378	WP_019727137.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-101 / qacE / sul1 / aac(6')-Ib / blaTEM-1B / blaCTX-M-30	-	1..59923	59923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (11-48)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10779.36 Da
• Isoelectric Point: 7.1659

>T89668 WP_019727125.1 NZ_CP025052:c9434-9159 [Pseudomonas aeruginosa]
MELKWTSKGLSDVARLYEFLATVNREAAARTVQQLTSAPTALLTNPRLGERLEEFEPRDVRRILIGHYEMRYEILDSCIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T89668 NZ_CP025052:c9434-9159 [Pseudomonas aeruginosa]
ATGGAGTTGAAGTGGACGAGCAAAGGGCTCTCCGACGTGGCACGGCTCTACGAGTTCCTGGCTACGGTGAACAGGGAAGC
TGCCGCCCGCACGGTGCAGCAGCTGACGAGCGCGCCGACCGCCTTGTTGACCAACCCGCGGCTGGGCGAGCGCCTGGAAG
AGTTCGAACCGCGGGATGTTCGCCGCATCCTGATCGGCCACTATGAGATGCGCTATGAGATCCTGGATTCCTGCATCTAC
GTGCTTCGTCTGTGGCACACACGCGAAGACAGGTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10142.32 Da
• Isoelectric Point: 4.8433

>AT89668 WP_019727126.1 NZ_CP025052:c9709-9434 [Pseudomonas aeruginosa]
MSTAAKPTRTVTAHVPVDLAERVDAIAERLERSKNWIVRQALSAWIDQEDERSRLTREALDDVDAGRVVDHQAVQAWAES
LGTDSPLPVPR

Nucleotide

• Length: 276 bp

>AT89668 NZ_CP025052:c9709-9434 [Pseudomonas aeruginosa]
ATGAGCACAGCAGCCAAACCAACGCGCACCGTGACTGCTCACGTTCCGGTAGATCTGGCCGAGCGCGTCGATGCAATCGC
CGAGCGCCTGGAGCGTTCGAAGAACTGGATCGTCAGGCAGGCGCTGTCAGCCTGGATCGACCAGGAAGACGAGCGTAGCC
GTCTGACGCGAGAGGCCCTGGATGATGTAGACGCAGGACGTGTCGTCGATCACCAGGCCGTACAGGCCTGGGCCGAAAGC
CTGGGCACTGACTCTCCGCTGCCGGTACCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B1YL09