Detailed information of TA system TA089641

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3138775..3139610
• Replicon: chromosome
• Accession: NZ_CP025048
• Organism: Escherichia coli strain SC516

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CWI33_RS15850
• Protein ID: WP_057109034.1
• Coordinates: 3138775..3139152 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1E5MC43
• Locus tag: CWI33_RS15855
• Protein ID: WP_063112944.1
• Coordinates: 3139242..3139610 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CWI33_RS15825	3135142..3136680	-	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-
CWI33_RS15835	3137429..3138271	-	843	WP_001529559.1	DUF4942 domain-containing protein	-
CWI33_RS15840	3138356..3138553	-	198	WP_023563519.1	DUF957 domain-containing protein	-
CWI33_RS15845	3138629..3138778	-	150	Protein_2895	hypothetical protein	-
CWI33_RS15850	3138775..3139152	-	378	WP_057109034.1	TA system toxin CbtA family protein	Toxin
CWI33_RS15855	3139242..3139610	-	369	WP_063112944.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CWI33_RS15860	3139660..3140304	-	645	WP_045146861.1	hypothetical protein	-
CWI33_RS15865	3140323..3140544	-	222	WP_000692323.1	DUF987 domain-containing protein	-
CWI33_RS15870	3140607..3141083	-	477	WP_001186780.1	RadC family protein	-
CWI33_RS15875	3141099..3141584	-	486	WP_000213716.1	antirestriction protein	-
CWI33_RS15880	3141676..3142493	-	818	Protein_2902	DUF932 domain-containing protein	-
CWI33_RS24770	3142594..3142746	-	153	WP_001696589.1	DUF905 family protein	-
CWI33_RS15885	3142752..3143429	-	678	WP_063073422.1	hypothetical protein	-
CWI33_RS15890	3143577..3144257	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3127653..3169452	41799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13934.86 Da
• Isoelectric Point: 6.8517

>T89641 WP_057109034.1 NZ_CP025048:c3139152-3138775 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T89641 NZ_CP025048:c3139152-3138775 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13724.52 Da
• Isoelectric Point: 6.8270

>AT89641 WP_063112944.1 NZ_CP025048:c3139610-3139242 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT89641 NZ_CP025048:c3139610-3139242 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCACGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1E5MC43