Detailed information of TA system TA089456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1105347..1105873
• Replicon: chromosome
• Accession: NZ_CP025003
• Organism: Dickeya fangzhongdai strain DSM 101947

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: C6CCH1
• Locus tag: CVE23_RS05090
• Protein ID: WP_015854715.1
• Coordinates: 1105347..1105652 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: CVE23_RS05095
• Protein ID: WP_100849039.1
• Coordinates: 1105655..1105873 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CVE23_RS05080	1101950..1103959	+	2010	WP_188726081.1	ATP-dependent helicase	-
CVE23_RS05085	1103961..1105220	+	1260	WP_100849038.1	GIY-YIG nuclease family protein	-
CVE23_RS05090	1105347..1105652	-	306	WP_015854715.1	type II toxin-antitoxin system toxin CcdB	Toxin
CVE23_RS05095	1105655..1105873	-	219	WP_100849039.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CVE23_RS05100	1105932..1106675	-	744	WP_100849040.1	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
CVE23_RS05110	1107577..1107927	-	351	WP_100849042.1	hypothetical protein	-
CVE23_RS22665	1108050..1108238	+	189	WP_033111580.1	hypothetical protein	-
CVE23_RS05115	1108391..1109653	-	1263	WP_100849043.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1091458..1109830	18372

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11676.54 Da
• Isoelectric Point: 4.8616

>T89456 WP_015854715.1 NZ_CP025003:c1105652-1105347 [Dickeya fangzhongdai]
MQFIVYEYKRASHYKMFVDVQSDIVETPKRRMAIPLIESHHLSEKVNKTLFPLIRIDGEDYRLMTTELSSVPVEVIGEVI
ADLGGYADEIKDAINLMFWGI

Nucleotide

• Length: 306 bp

>T89456 NZ_CP025003:c1105652-1105347 [Dickeya fangzhongdai]
ATGCAATTCATTGTTTATGAATACAAACGCGCCAGTCATTACAAAATGTTTGTCGATGTGCAAAGTGATATTGTCGAGAC
TCCCAAGCGGCGTATGGCTATCCCGCTTATCGAATCGCACCACCTGTCTGAGAAAGTGAATAAAACGCTGTTCCCGCTGA
TTCGCATCGATGGGGAAGATTATCGGCTGATGACCACTGAGCTATCGAGCGTTCCTGTTGAGGTCATTGGTGAAGTTATC
GCGGATCTTGGCGGCTACGCAGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8137.12 Da
• Isoelectric Point: 8.6801

>AT89456 WP_100849039.1 NZ_CP025003:c1105873-1105655 [Dickeya fangzhongdai]
MKHRVSVTVDKDNYQVLSAAGVNISGLVNDAIGKEARRIKAEAWRKENREGMEEVARLIAQHGSFADENRNW

Nucleotide

• Length: 219 bp

>AT89456 NZ_CP025003:c1105873-1105655 [Dickeya fangzhongdai]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAATTATCAGGTTCTGAGTGCTGCCGGAGTCAATATTTCCGGGCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGCATCAAAGCTGAAGCGTGGAGAAAGGAAAACCGCGAAGGGATGGAGG
AAGTCGCCCGACTCATCGCACAGCATGGCTCCTTTGCGGATGAAAACAGGAACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2K8QIT0