Detailed information of TA system TA089378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 19580..20139
• Replicon: plasmid pCV839-15-p2
• Accession: NZ_CP024976
• Organism: Escherichia coli strain CV839-15

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: CV83915_RS00745
• Protein ID: WP_000421259.1
• Coordinates: 19864..20139 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: CV83915_RS00740
• Protein ID: WP_001178087.1
• Coordinates: 19580..19864 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83915_RS00690	15228..15761	-	534	WP_001372168.1	transcription termination factor NusG	-
CV83915_RS00700	16203..16490	-	288	WP_000074849.1	conjugal transfer protein TraA	-
CV83915_RS00705	16408..16671	-	264	WP_001324596.1	ash family protein	-
CV83915_RS00720	17644..18675	+	1032	WP_103758514.1	replication initiation protein	-
CV83915_RS00740	19580..19864	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
CV83915_RS00745	19864..20139	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CV83915_RS00750	20245..20526	+	282	WP_001105066.1	hypothetical protein	-
CV83915_RS00760	20950..23835	-	2886	WP_001138082.1	Tn3 family transposase	-
CV83915_RS00765	23970..24584	+	615	WP_010981357.1	recombinase family protein	-
CV83915_RS00770	24623..24943	-	321	WP_010981356.1	DUF3330 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2	-	1..195578	195578

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T89378 WP_000421259.1 NZ_CP024976:19864-20139 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T89378 NZ_CP024976:19864-20139 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT89378 WP_001178087.1 NZ_CP024976:19580-19864 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT89378 NZ_CP024976:19580-19864 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625