Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4998032..4998678 Replicon chromosome
Accession NZ_CP024916
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name higB Uniprot ID A0A4S7KZ03
Locus tag CU643_RS25145 Protein ID WP_032433636.1
Coordinates 4998331..4998678 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag CU643_RS25140 Protein ID WP_019725405.1
Coordinates 4998032..4998331 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS25120 4994238..4994996 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
CU643_RS25125 4995046..4995876 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
CU643_RS25130 4995927..4996256 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
CU643_RS25135 4996461..4997969 + 1509 WP_029602548.1 glycerol-3-phosphate dehydrogenase -
CU643_RS25140 4998032..4998331 - 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
CU643_RS25145 4998331..4998678 - 348 WP_032433636.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CU643_RS25150 4998854..5001301 - 2448 WP_032433638.1 glycogen phosphorylase -
CU643_RS25155 5001319..5002752 - 1434 WP_032433640.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13500.51 Da        Isoelectric Point: 6.2327

>T89274 WP_032433636.1 NZ_CP024916:c4998678-4998331 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGINEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T89274 NZ_CP024916:c4998678-4998331 [Klebsiella pneumoniae]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT89274 WP_019725405.1 NZ_CP024916:c4998331-4998032 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT89274 NZ_CP024916:c4998331-4998032 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S7KZ03

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References