Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3881545..3882281 Replicon chromosome
Accession NZ_CP024916
Organism Klebsiella pneumoniae strain NH54

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag CU643_RS19360 Protein ID WP_032433360.1
Coordinates 3881545..3882027 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag CU643_RS19365 Protein ID WP_003026799.1
Coordinates 3882015..3882281 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU643_RS19335 3876555..3877133 + 579 WP_032432061.1 type 1 fimbrial protein -
CU643_RS19340 3877145..3878089 + 945 WP_077254249.1 fimbrial protein -
CU643_RS19345 3878302..3878901 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
CU643_RS19350 3879281..3879913 + 633 WP_001567369.1 hypothetical protein -
CU643_RS19355 3879942..3881345 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
CU643_RS19360 3881545..3882027 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
CU643_RS19365 3882015..3882281 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
CU643_RS19370 3882594..3883157 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
CU643_RS19375 3883167..3883799 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
CU643_RS19380 3883817..3884821 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
CU643_RS19385 3884805..3885467 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
CU643_RS19390 3885496..3886431 - 936 WP_199853646.1 mannitol dehydrogenase -
CU643_RS29340 3886464..3886634 - 171 WP_199853647.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3879942..3881345 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T89271 WP_032433360.1 NZ_CP024916:c3882027-3881545 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T89271 NZ_CP024916:c3882027-3881545 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT89271 WP_003026799.1 NZ_CP024916:c3882281-3882015 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT89271 NZ_CP024916:c3882281-3882015 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References