Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 453501..454138 Replicon chromosome
Accession NZ_CP024897
Organism Bacillus velezensis strain CN026

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag CVD07_RS02290 Protein ID WP_003156187.1
Coordinates 453788..454138 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag CVD07_RS02285 Protein ID WP_003156188.1
Coordinates 453501..453782 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CVD07_RS02265 449866..450465 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
CVD07_RS02270 450558..450923 + 366 WP_014304402.1 holo-ACP synthase -
CVD07_RS02275 451088..452095 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
CVD07_RS02280 452212..453381 + 1170 WP_021495079.1 alanine racemase -
CVD07_RS02285 453501..453782 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
CVD07_RS02290 453788..454138 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CVD07_RS02295 454256..455077 + 822 WP_003156182.1 STAS domain-containing protein -
CVD07_RS02300 455082..455447 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
CVD07_RS02305 455450..455851 + 402 WP_003156178.1 anti-sigma regulatory factor -
CVD07_RS02310 455863..456870 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
CVD07_RS02315 456934..457263 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
CVD07_RS02320 457260..457742 + 483 WP_007609591.1 anti-sigma B factor RsbW -
CVD07_RS02325 457708..458496 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
CVD07_RS02330 458496..459098 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T89157 WP_003156187.1 NZ_CP024897:453788-454138 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T89157 NZ_CP024897:453788-454138 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT89157 WP_003156188.1 NZ_CP024897:453501-453782 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT89157 NZ_CP024897:453501-453782 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References