Detailed information of TA system TA088936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 14855..15447
• Replicon: plasmid unitig_2_pilon
• Accession: NZ_CP024861
• Organism: Escherichia coli strain AR_0014

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: AM341_RS25980
• Protein ID: WP_000520549.1
• Coordinates: 14855..15103 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: AM341_RS25985
• Protein ID: WP_000681613.1
• Coordinates: 15100..15447 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM341_RS25930	10112..10447	+	336	WP_001147119.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AM341_RS25935	10444..10794	+	351	WP_000127482.1	addiction module antitoxin	-
AM341_RS26485	11434..11949	+	516	WP_001025395.1	J domain-containing protein	-
AM341_RS25945	12054..12596	+	543	WP_001083906.1	hypothetical protein	-
AM341_RS25950	12593..12841	+	249	WP_001230706.1	hypothetical protein	-
AM341_RS25955	12831..13058	+	228	WP_000801440.1	hypothetical protein	-
AM341_RS25965	13512..13811	+	300	WP_000650311.1	hypothetical protein	-
AM341_RS25970	13821..14237	+	417	WP_012421208.1	tail-associated lysozyme	-
AM341_RS25975	14234..14545	+	312	WP_000627398.1	hypothetical protein	-
AM341_RS25980	14855..15103	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
AM341_RS25985	15100..15447	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
AM341_RS25995	15810..16313	+	504	WP_000781814.1	hypothetical protein	-
AM341_RS26000	16317..17546	+	1230	WP_016231858.1	relaxase/mobilization nuclease domain-containing protein	-
AM341_RS26005	17623..17916	+	294	WP_000134733.1	LuxR family transcriptional regulator	-
AM341_RS26010	17913..18128	+	216	WP_000760375.1	EexN family lipoprotein	-
AM341_RS26015	18485..19105	+	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
AM341_RS26020	19173..19445	+	273	WP_001353733.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33548	33548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T88936 WP_000520549.1 NZ_CP024861:14855-15103 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T88936 NZ_CP024861:14855-15103 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT88936 WP_000681613.1 NZ_CP024861:15100-15447 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT88936 NZ_CP024861:15100-15447 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1