Detailed information of TA system TA088856

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101696..102122
• Replicon: plasmid tig00000164
• Accession: NZ_CP024852
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AM333_RS26595
• Protein ID: WP_001312861.1
• Coordinates: 101696..101854 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	101898..102122 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS26575	97206..98027	-	822	WP_001234426.1	DUF945 domain-containing protein	-
AM333_RS26580	98145..98432	-	288	WP_000107535.1	hypothetical protein	-
AM333_RS26590	99634..101301	+	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
AM333_RS26595	101696..101854	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	101898..102122	-	225	NuclAT_0	-	Antitoxin
-	101898..102122	-	225	NuclAT_0	-	Antitoxin
-	101898..102122	-	225	NuclAT_0	-	Antitoxin
-	101898..102122	-	225	NuclAT_0	-	Antitoxin
AM333_RS28215	101934..102122	+	189	WP_001299721.1	hypothetical protein	-
AM333_RS26600	102134..102853	-	720	WP_013362833.1	plasmid SOS inhibition protein A	-
AM333_RS26605	102850..103284	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
AM333_RS26610	103339..105297	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
AM333_RS26615	105356..105589	-	234	WP_000006004.1	DUF905 domain-containing protein	-
AM333_RS26625	105954..106775	+	822	WP_001272932.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 / qacE / cmlA1 / aac(3)-IIa / mph(A) / sul1 / aadA5 / dfrA17 / blaTEM-1B / sitABCD	-	1..167974	167974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88856 WP_001312861.1 NZ_CP024852:c101854-101696 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88856 NZ_CP024852:c101854-101696 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT88856 NZ_CP024852:c102122-101898 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin