Detailed information of TA system TA088852

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59268..59522
• Replicon: plasmid tig00000164
• Accession: NZ_CP024852
• Organism: Escherichia coli strain AR_0006

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AM333_RS26340
• Protein ID: WP_015387399.1
• Coordinates: 59268..59474 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	59461..59522 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM333_RS26305	54515..55426	-	912	WP_000440183.1	carbamate kinase	-
AM333_RS26310	55437..56657	-	1221	WP_000410951.1	arginine deiminase	-
AM333_RS26315	57550..58032	+	483	Protein_70	VENN motif pre-toxin domain-containing protein	-
AM333_RS26320	57978..58424	-	447	Protein_71	incFII family plasmid replication initiator RepA	-
AM333_RS26325	58417..58491	-	75	WP_032336874.1	RepA leader peptide Tap	-
AM333_RS26335	58727..58984	-	258	WP_000083833.1	replication regulatory protein RepA	-
AM333_RS26340	59268..59474	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	59461..59522	+	62	NuclAT_1	-	Antitoxin
-	59461..59522	+	62	NuclAT_1	-	Antitoxin
-	59461..59522	+	62	NuclAT_1	-	Antitoxin
-	59461..59522	+	62	NuclAT_1	-	Antitoxin
AM333_RS26350	59661..59843	-	183	WP_000968309.1	hypothetical protein	-
AM333_RS26360	59944..60560	+	617	Protein_76	IS1 family transposase	-
AM333_RS26365	60598..62169	-	1572	WP_100190670.1	IS66 family transposase	-
AM333_RS26370	62189..62536	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
AM333_RS26375	62536..63213	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
AM333_RS28400	63268..63357	+	90	Protein_80	IS1 family transposase	-
AM333_RS26390	63658..63870	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 / qacE / cmlA1 / aac(3)-IIa / mph(A) / sul1 / aadA5 / dfrA17 / blaTEM-1B / sitABCD	-	1..167974	167974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T88852 WP_015387399.1 NZ_CP024852:c59474-59268 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T88852 NZ_CP024852:c59474-59268 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT88852 NZ_CP024852:59461-59522 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin