Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29303..29826 Replicon plasmid pCRKP-2297_1
Accession NZ_CP024835
Organism Klebsiella pneumoniae strain CRKP-2297

Toxin (Protein)


Gene name ccdB Uniprot ID V5RNQ6
Locus tag CU075_RS27510 Protein ID WP_015632464.1
Coordinates 29521..29826 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V5RQR8
Locus tag CU075_RS27505 Protein ID WP_015632465.1
Coordinates 29303..29521 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU075_RS27485 25446..26717 - 1272 WP_016162068.1 Y-family DNA polymerase -
CU075_RS27490 26717..27148 - 432 WP_015632467.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
CU075_RS27495 27312..27797 - 486 Protein_29 IS5 family transposase -
CU075_RS27500 28092..29117 - 1026 WP_015632466.1 IS110 family transposase -
CU075_RS27505 29303..29521 + 219 WP_015632465.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CU075_RS27510 29521..29826 + 306 WP_015632464.1 type II toxin-antitoxin system toxin CcdB Toxin
CU075_RS27515 29971..30315 + 345 WP_015632463.1 hypothetical protein -
CU075_RS27520 30357..30923 + 567 WP_015632462.1 hypothetical protein -
CU075_RS27525 31127..31384 - 258 WP_016162071.1 helix-turn-helix domain-containing protein -
CU075_RS27530 31814..32041 + 228 WP_016162072.1 hypothetical protein -
CU075_RS29255 32055..32177 + 123 Protein_37 transcriptional regulator -
CU075_RS27540 32383..32826 - 444 WP_015632459.1 type II toxin-antitoxin system HicB family antitoxin -
CU075_RS27545 32852..33034 - 183 WP_015632458.1 type II toxin-antitoxin system HicA family toxin -
CU075_RS27550 33395..34375 + 981 WP_015632457.1 partitioning protein -
CU075_RS27555 34368..34796 + 429 WP_015632456.1 plasmid partitioning/stability family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ARR-3 / qnrB1 - 1..112150 112150


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11361.90 Da        Isoelectric Point: 5.5252

>T88765 WP_015632464.1 NZ_CP024835:29521-29826 [Klebsiella pneumoniae]
MQFTVYEYTRESHYRLFVDVQSDIVETPGRRIVIPLLESHHLSEKVNQQLFPVVSVNGKEYRAVTSELSGVSVSVIGDAV
ADLSHEANAIKNALNLMFWGI

Download         Length: 306 bp

>T88765 NZ_CP024835:29521-29826 [Klebsiella pneumoniae]
ATGCAGTTCACCGTCTACGAATACACACGCGAGAGCCATTATCGTCTTTTTGTGGATGTGCAGAGCGACATTGTCGAGAC
GCCTGGGCGACGCATAGTGATCCCGTTGCTGGAATCGCACCACTTATCAGAAAAGGTTAATCAGCAGCTTTTCCCGGTGG
TTAGCGTCAATGGGAAAGAATACCGTGCCGTGACTTCCGAATTATCCGGAGTCTCTGTCAGTGTCATCGGCGACGCTGTG
GCCGATCTCAGCCATGAGGCAAATGCCATTAAAAATGCCCTGAACCTGATGTTCTGGGGCATTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8194.13 Da        Isoelectric Point: 6.2224

>AT88765 WP_015632465.1 NZ_CP024835:29303-29521 [Klebsiella pneumoniae]
MKHRASVTVDKEHYLELLAAGVNISGLVNEAIGREARRIQAERWKAENREGMEEVANFIAQHGSFADENRSW

Download         Length: 219 bp

>AT88765 NZ_CP024835:29303-29521 [Klebsiella pneumoniae]
ATGAAACACAGAGCCAGCGTCACAGTAGACAAGGAACATTATCTGGAATTACTCGCGGCAGGCGTGAATATTTCGGGACT
GGTGAATGAGGCTATCGGACGGGAAGCCCGGCGCATCCAGGCTGAACGCTGGAAAGCAGAGAACCGGGAAGGGATGGAAG
AGGTAGCGAATTTCATCGCGCAGCACGGCTCTTTTGCCGACGAAAACCGGAGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0ND19

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0L1T7

References