Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3397908..3398434 Replicon chromosome
Accession NZ_CP024830
Organism Escherichia coli strain CREC-532

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag CU076_RS17535 Protein ID WP_000323025.1
Coordinates 3397908..3398195 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag CU076_RS17540 Protein ID WP_000534858.1
Coordinates 3398195..3398434 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CU076_RS17495 3392932..3393147 - 216 WP_000839590.1 phage lysis protein EssD -
CU076_RS28065 3393367..3393537 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
CU076_RS17500 3393901..3394116 - 216 WP_000066484.1 cold shock-like protein CspB -
CU076_RS17505 3394417..3394629 + 213 WP_000087756.1 cold shock-like protein CspF -
CU076_RS27500 3394684..3394773 + 90 WP_120795389.1 hypothetical protein -
CU076_RS17510 3395051..3395803 - 753 WP_001047135.1 antitermination protein -
CU076_RS17515 3395817..3396866 - 1050 WP_001265199.1 DUF968 domain-containing protein -
CU076_RS17520 3396868..3397146 - 279 WP_012304870.1 hypothetical protein -
CU076_RS17525 3397213..3397464 - 252 WP_000980994.1 hypothetical protein -
CU076_RS17530 3397681..3397836 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
CU076_RS17535 3397908..3398195 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
CU076_RS17540 3398195..3398434 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
CU076_RS17545 3398459..3398764 + 306 WP_001326990.1 hypothetical protein -
CU076_RS17550 3398967..3399299 + 333 WP_001301033.1 protein FlxA -
CU076_RS17560 3399736..3401049 - 1314 WP_000589005.1 ISNCY family transposase -
CU076_RS27785 3401818..3402105 - 288 Protein_3192 hypothetical protein -
CU076_RS17575 3402327..3402425 + 99 Protein_3193 IS66 family insertion sequence element accessory protein TnpB -
CU076_RS17580 3402473..3402706 + 234 WP_001341330.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3382720..3409219 26499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T88723 WP_000323025.1 NZ_CP024830:c3398195-3397908 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T88723 NZ_CP024830:c3398195-3397908 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT88723 WP_000534858.1 NZ_CP024830:c3398434-3398195 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT88723 NZ_CP024830:c3398434-3398195 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References