Detailed information of TA system TA088709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4075..4721
• Replicon: plasmid pCREC-544_3
• Accession: NZ_CP024829
• Organism: Escherichia coli strain CREC-544

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CU077_RS25910
• Protein ID: WP_000269912.1
• Coordinates: 4374..4721 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CU077_RS25905
• Protein ID: WP_001673379.1
• Coordinates: 4075..4374 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU077_RS26490	383..556	+	174	WP_157838146.1	ash family protein	-
CU077_RS25875	634..825	+	192	WP_000183351.1	hypothetical protein	-
CU077_RS25880	822..1964	+	1143	WP_021542821.1	ORF6N domain-containing protein	-
CU077_RS25890	2773..3045	-	273	WP_000148348.1	helix-turn-helix domain-containing protein	-
CU077_RS25895	3109..3489	-	381	WP_000061763.1	hypothetical protein	-
CU077_RS25900	3757..4041	+	285	WP_021542820.1	hypothetical protein	-
CU077_RS25905	4075..4374	-	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
CU077_RS25910	4374..4721	-	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CU077_RS25915	4965..5228	-	264	WP_000424604.1	hypothetical protein	-
CU077_RS25920	5252..5539	-	288	WP_000356589.1	hypothetical protein	-
CU077_RS25925	6183..7019	-	837	WP_024189035.1	replication initiator protein RepA	-
CU077_RS25930	7345..7899	-	555	WP_100183613.1	recombinase family protein	-
CU077_RS25940	8035..8877	+	843	WP_024133807.1	tail fiber protein	-
CU077_RS25945	8879..9406	+	528	WP_000972114.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32182	32182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T88709 WP_000269912.1 NZ_CP024829:c4721-4374 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T88709 NZ_CP024829:c4721-4374 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAACGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT88709 WP_001673379.1 NZ_CP024829:c4374-4075 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT88709 NZ_CP024829:c4374-4075 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCTACAGGAAAGCGAGTAGCGTTCCATGTCTAA