Detailed information of TA system TA088695

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4069302..4070100
• Replicon: chromosome
• Accession: NZ_CP024826
• Organism: Escherichia coli strain CREC-544

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1M0HL60
• Locus tag: CU077_RS20500
• Protein ID: WP_000854692.1
• Coordinates: 4069723..4070100 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1L4NTN5
• Locus tag: CU077_RS20495
• Protein ID: WP_001605875.1
• Coordinates: 4069302..4069676 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU077_RS20475	4067061..4067879	+	819	WP_001605871.1	DUF945 domain-containing protein	-
CU077_RS20480	4067961..4068440	+	480	WP_001605873.1	antirestriction protein	-
CU077_RS20485	4068456..4068932	+	477	WP_001605874.1	RadC family protein	-
CU077_RS20490	4069001..4069222	+	222	WP_000692303.1	DUF987 domain-containing protein	-
CU077_RS20495	4069302..4069676	+	375	WP_001605875.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
CU077_RS20500	4069723..4070100	+	378	WP_000854692.1	TA system toxin CbtA family protein	Toxin
CU077_RS26280	4070097..4070246	+	150	Protein_3783	hypothetical protein	-
CU077_RS20505	4070322..4070519	+	198	WP_023563519.1	DUF957 domain-containing protein	-
CU077_RS20510	4070604..4071446	+	843	WP_001529559.1	DUF4942 domain-containing protein	-
CU077_RS20515	4071789..4071959	+	171	Protein_3786	IS110 family transposase	-
CU077_RS20520	4072770..4073753	+	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
CU077_RS20525	4073825..4074973	+	1149	WP_000905931.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sfaX / kpsF / kpsE / kpsD	4025990..4076673	50683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14119.12 Da
• Isoelectric Point: 7.3249

>T88695 WP_000854692.1 NZ_CP024826:4069723-4070100 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWHTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T88695 NZ_CP024826:4069723-4070100 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13594.42 Da
• Isoelectric Point: 5.4554

>AT88695 WP_001605875.1 NZ_CP024826:4069302-4069676 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLACEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT88695 NZ_CP024826:4069302-4069676 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A1M0HL60

Antitoxin

• AlphaFold DB: A0A1L4NTN5