Detailed information of TA system TA088665

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13465..13718
• Replicon: plasmid pCREC-591_2
• Accession: NZ_CP024823
• Organism: Escherichia coli strain CREC-591

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CU078_RS27705
• Protein ID: WP_001312851.1
• Coordinates: 13569..13718 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	13465..13524 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CU078_RS27675	10192..10938	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CU078_RS27680	10993..11553	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CU078_RS27685	11685..11885	+	201	WP_015059022.1	hypothetical protein	-
CU078_RS27690	12271..12870	+	600	WP_032083981.1	hypothetical protein	-
CU078_RS27695	13034..13264	+	231	WP_001736714.1	hypothetical protein	-
-	13465..13524	-	60	NuclAT_1	-	Antitoxin
-	13465..13524	-	60	NuclAT_1	-	Antitoxin
-	13465..13524	-	60	NuclAT_1	-	Antitoxin
-	13465..13524	-	60	NuclAT_1	-	Antitoxin
CU078_RS27705	13569..13718	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CU078_RS27710	14002..14250	+	249	WP_000083837.1	replication regulatory protein RepA	-
CU078_RS27720	14495..14569	+	75	WP_001375168.1	RepA leader peptide Tap	-
CU078_RS27725	14562..15419	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
CU078_RS29485	16120..16260	+	141	WP_001333237.1	hypothetical protein	-
CU078_RS27735	16358..17011	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
CU078_RS29225	17104..17361	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
CU078_RS27740	17294..17695	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
CU078_RS27750	17944..18359	+	416	Protein_19	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..64564	64564

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T88665 WP_001312851.1 NZ_CP024823:13569-13718 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T88665 NZ_CP024823:13569-13718 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT88665 NZ_CP024823:c13524-13465 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin