Detailed information of TA system TA088456

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100440..100866
• Replicon: plasmid p1LS4
• Accession: NZ_CP024718
• Organism: Escherichia coli strain LS4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CUB99_RS27680
• Protein ID: WP_001312861.1
• Coordinates: 100440..100598 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	100642..100866 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CUB99_RS27630	95478..95705	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
CUB99_RS27635	95799..96485	-	687	WP_000332487.1	PAS domain-containing protein	-
CUB99_RS27640	96679..97062	-	384	WP_001151564.1	relaxosome protein TraM	-
CUB99_RS27645	97348..97995	+	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
CUB99_RS27650	98292..99113	-	822	WP_001234469.1	DUF945 domain-containing protein	-
CUB99_RS27655	99234..99521	-	288	WP_099996262.1	hypothetical protein	-
CUB99_RS27680	100440..100598	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	100642..100866	-	225	NuclAT_0	-	Antitoxin
-	100642..100866	-	225	NuclAT_0	-	Antitoxin
-	100642..100866	-	225	NuclAT_0	-	Antitoxin
-	100642..100866	-	225	NuclAT_0	-	Antitoxin
CUB99_RS28080	100678..100866	+	189	WP_001299721.1	hypothetical protein	-
CUB99_RS27685	100878..101597	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
CUB99_RS27690	101594..102028	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
CUB99_RS27695	102083..104041	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
CUB99_RS27700	104100..104333	-	234	WP_000006004.1	DUF905 domain-containing protein	-
CUB99_RS27705	104389..104916	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aadA5 / qacE / sul1	-	1..111779	111779

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T88456 WP_001312861.1 NZ_CP024718:c100598-100440 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T88456 NZ_CP024718:c100598-100440 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT88456 NZ_CP024718:c100866-100642 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin