Detailed information of TA system TA088088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3852402..3853129
• Replicon: chromosome
• Accession: NZ_CP024618
• Organism: Escherichia coli strain SMN152SH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: CR916_RS22380
• Protein ID: WP_000547563.1
• Coordinates: 3852818..3853129 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CR916_RS22375
• Protein ID: WP_000126296.1
• Coordinates: 3852402..3852821 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CR916_RS22355	3847452..3847979	-	528	WP_001078777.1	electron transport protein HydN	-
CR916_RS22360	3848128..3849138	-	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
CR916_RS22365	3849398..3850855	+	1458	WP_001107859.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CR916_RS22370	3850864..3852288	+	1425	WP_024226292.1	6-phospho-beta-glucosidase AscB	-
CR916_RS22375	3852402..3852821	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
CR916_RS22380	3852818..3853129	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
CR916_RS22385	3853291..3854042	-	752	Protein_3960	hypothetical protein	-
CR916_RS22390	3854085..3854555	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CR916_RS22395	3854548..3854958	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CR916_RS22400	3854955..3855722	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
CR916_RS22405	3855722..3856264	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CR916_RS22410	3856274..3857983	-	1710	WP_062881985.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T88088 WP_000547563.1 NZ_CP024618:c3853129-3852818 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T88088 NZ_CP024618:c3853129-3852818 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT88088 WP_000126296.1 NZ_CP024618:c3852821-3852402 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT88088 NZ_CP024618:c3852821-3852402 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22