Detailed information of TA system TA087949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3917013..3917610
• Replicon: chromosome
• Accession: NZ_CP024548
• Organism: Klebsiella pneumoniae strain KSB1_7J

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: CTI66_RS19785
• Protein ID: WP_004893639.1
• Coordinates: 3917293..3917610 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: CTI66_RS19780
• Protein ID: WP_004142561.1
• Coordinates: 3917013..3917300 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CTI66_RS19750	3913220..3913468	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
CTI66_RS19755	3913486..3913827	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
CTI66_RS19760	3913858..3914973	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
CTI66_RS19765	3915153..3915734	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
CTI66_RS19770	3915734..3916102	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
CTI66_RS19775	3916222..3916875	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
CTI66_RS19780	3917013..3917300	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
CTI66_RS19785	3917293..3917610	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CTI66_RS19790	3917795..3918838	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
CTI66_RS19800	3919508..3920374	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
CTI66_RS19805	3920483..3921910	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T87949 WP_004893639.1 NZ_CP024548:c3917610-3917293 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T87949 NZ_CP024548:c3917610-3917293 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT87949 WP_004142561.1 NZ_CP024548:c3917300-3917013 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT87949 NZ_CP024548:c3917300-3917013 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1