Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1739..2298 Replicon plasmid unnamed2
Accession NZ_CP024481
Organism Escherichia coli strain 2011C-4315

Toxin (Protein)


Gene name relE Uniprot ID A0A733EV64
Locus tag A5958_RS28895 Protein ID WP_001582571.1
Coordinates 2023..2298 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag A5958_RS28890 Protein ID WP_001178089.1
Coordinates 1739..2023 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A5958_RS28890 1739..2023 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
A5958_RS28895 2023..2298 + 276 WP_001582571.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A5958_RS28900 2404..2682 + 279 WP_001105069.1 hypothetical protein -
A5958_RS30130 2877..3143 - 267 WP_000627829.1 hypothetical protein -
A5958_RS28910 3143..3637 - 495 WP_001582574.1 hypothetical protein -
A5958_RS28915 3693..4295 - 603 WP_000517695.1 hypothetical protein -
A5958_RS28925 4649..5995 + 1347 WP_001132032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..105489 105489


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10807.48 Da        Isoelectric Point: 9.5296

>T87782 WP_001582571.1 NZ_CP024481:2023-2298 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNDQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T87782 NZ_CP024481:2023-2298 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGACCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT87782 WP_001178089.1 NZ_CP024481:1739-2023 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT87782 NZ_CP024481:1739-2023 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A733EV64

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References