Detailed information of TA system TA087581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2620008..2620845
• Replicon: chromosome
• Accession: NZ_CP024289
• Organism: Escherichia coli O178:H19 strain 2012C-4431

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: A5960_RS13885
• Protein ID: WP_000227784.1
• Coordinates: 2620303..2620845 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: A5960_RS13880
• Protein ID: WP_001297137.1
• Coordinates: 2620008..2620319 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A5960_RS13855	2615028..2615975	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
A5960_RS13860	2615997..2617988	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
A5960_RS13865	2617978..2618592	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
A5960_RS13870	2618592..2618921	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
A5960_RS13875	2618933..2619823	+	891	WP_000971336.1	heme o synthase	-
A5960_RS13880	2620008..2620319	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
A5960_RS13885	2620303..2620845	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
A5960_RS13890	2620901..2621836	-	936	WP_001368479.1	sel1 repeat family protein	-
A5960_RS13895	2622244..2623608	+	1365	WP_001000960.1	MFS transporter	-
A5960_RS13900	2623736..2624227	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
A5960_RS13905	2624395..2625306	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T87581 WP_000227784.1 NZ_CP024289:2620303-2620845 [Escherichia coli O178:H19]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T87581 NZ_CP024289:2620303-2620845 [Escherichia coli O178:H19]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT87581 WP_001297137.1 NZ_CP024289:2620008-2620319 [Escherichia coli O178:H19]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT87581 NZ_CP024289:2620008-2620319 [Escherichia coli O178:H19]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3