Detailed information of TA system TA087493

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1487675..1488507
• Replicon: chromosome
• Accession: NZ_CP024278
• Organism: Escherichia coli strain ATCC 43896

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: CS031_RS07730
• Protein ID: WP_000854762.1
• Coordinates: 1487675..1488049 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: CS031_RS07735
• Protein ID: WP_001295723.1
• Coordinates: 1488139..1488507 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS031_RS07705	1482891..1484399	-	1509	WP_001189123.1	group II intron reverse transcriptase/maturase	-
CS031_RS07715	1485072..1485811	-	740	Protein_1427	IS3 family transposase	-
CS031_RS07720	1485861..1487225	+	1365	Protein_1428	IS66-like element ISEc23 family transposase	-
CS031_RS27700	1487346..1487459	-	114	WP_001161660.1	DUF957 domain-containing protein	-
CS031_RS07725	1487472..1487678	-	207	WP_000976829.1	hypothetical protein	-
CS031_RS07730	1487675..1488049	-	375	WP_000854762.1	TA system toxin CbtA family protein	Toxin
CS031_RS07735	1488139..1488507	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
CS031_RS07740	1488670..1488891	-	222	WP_000692341.1	DUF987 domain-containing protein	-
CS031_RS07745	1488954..1489430	-	477	WP_001186774.1	RadC family protein	-
CS031_RS07750	1489446..1489919	-	474	WP_000855059.1	antirestriction protein	-
CS031_RS07760	1490261..1491079	-	819	WP_001234652.1	DUF945 domain-containing protein	-
CS031_RS07770	1491234..1491392	-	159	WP_001433433.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1460319..1494309	33990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14094.15 Da
• Isoelectric Point: 7.1881

>T87493 WP_000854762.1 NZ_CP024278:c1488049-1487675 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T87493 NZ_CP024278:c1488049-1487675 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT87493 WP_001295723.1 NZ_CP024278:c1488507-1488139 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT87493 NZ_CP024278:c1488507-1488139 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52