Detailed information of TA system TA087491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 859367..859992
• Replicon: chromosome
• Accession: NZ_CP024278
• Organism: Escherichia coli strain ATCC 43896

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U9YZ02
• Locus tag: CS031_RS04430
• Protein ID: WP_000911329.1
• Coordinates: 859594..859992 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CS031_RS04425
• Protein ID: WP_001696518.1
• Coordinates: 859367..859594 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CS031_RS04400	855170..855640	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
CS031_RS04405	855640..856212	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
CS031_RS04410	856358..857236	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CS031_RS04415	857253..858287	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
CS031_RS04420	858500..859213	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
CS031_RS04425	859367..859594	+	228	WP_001696518.1	toxin-antitoxin system antitoxin VapB	Antitoxin
CS031_RS04430	859594..859992	+	399	WP_000911329.1	tRNA(fMet)-specific endonuclease VapC	Toxin
CS031_RS04435	860139..861002	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
CS031_RS04440	861017..863032	+	2016	WP_001388162.1	tRNA cytosine(34) acetyltransferase TmcA	-
CS031_RS04445	863106..863804	+	699	WP_000679812.1	esterase	-
CS031_RS04450	863885..864085	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algU	718465..888157	169692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14924.23 Da
• Isoelectric Point: 8.5325

>T87491 WP_000911329.1 NZ_CP024278:859594-859992 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T87491 NZ_CP024278:859594-859992 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8526.66 Da
• Isoelectric Point: 4.3867

>AT87491 WP_001696518.1 NZ_CP024278:859367-859594 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIVIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT87491 NZ_CP024278:859367-859594 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGTGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0XW84