Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3870138..3870640 Replicon chromosome
Accession NZ_CP024256
Organism Escherichia coli strain ATCC 43886

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CS032_RS21960 Protein ID WP_000767829.1
Coordinates 3870138..3870392 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CS032_RS21965 Protein ID WP_001259255.1
Coordinates 3870389..3870640 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CS032_RS21930 3865153..3865380 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CS032_RS21935 3865394..3866452 - 1059 WP_000492340.1 transport protein YeeE -
CS032_RS21940 3866631..3867989 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CS032_RS21945 3867979..3868041 - 63 WP_010723108.1 membrane protein YoeI -
CS032_RS21950 3868256..3869185 - 930 WP_000803337.1 LysR family transcriptional regulator -
CS032_RS21955 3869231..3870055 - 825 WP_000754737.1 SDR family oxidoreductase -
CS032_RS21960 3870138..3870392 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CS032_RS21965 3870389..3870640 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CS032_RS21970 3870923..3870973 + 51 WP_001364200.1 his operon leader peptide -
CS032_RS21980 3871119..3872018 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CS032_RS21985 3872024..3873322 + 1299 WP_001735396.1 histidinol dehydrogenase -
CS032_RS21990 3873325..3874395 + 1071 WP_000108954.1 histidinol-phosphate transaminase -
CS032_RS21995 3874395..3875462 + 1068 WP_000080145.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T87308 WP_000767829.1 NZ_CP024256:c3870392-3870138 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87308 NZ_CP024256:c3870392-3870138 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87308 WP_001259255.1 NZ_CP024256:c3870640-3870389 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87308 NZ_CP024256:c3870640-3870389 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References