Detailed information of TA system TA087252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 135484..136127
• Replicon: plasmid unnamed
• Accession: NZ_CP024244
• Organism: Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: COG34_RS26720
• Protein ID: WP_001356337.1
• Coordinates: 135711..136127 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: COG34_RS26715
• Protein ID: WP_024220872.1
• Coordinates: 135484..135714 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG34_RS26690	130760..131404	-	645	WP_001144036.1	ParA family protein	-
COG34_RS26695	131585..132361	-	777	WP_001495932.1	site-specific integrase	-
COG34_RS26700	132412..133191	-	780	WP_000535296.1	hypothetical protein	-
COG34_RS26705	133244..133558	-	315	WP_001302628.1	hypothetical protein	-
COG34_RS26710	134184..135203	+	1020	Protein_157	IS110 family transposase	-
COG34_RS26715	135484..135714	+	231	WP_024220872.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
COG34_RS26720	135711..136127	+	417	WP_001356337.1	type II toxin-antitoxin system VapC family toxin	Toxin
COG34_RS26725	136094..136441	-	348	Protein_160	helix-turn-helix domain-containing protein	-
COG34_RS26740	137995..138270	+	276	Protein_162	integrase core domain-containing protein	-
COG34_RS26745	138279..138428	-	150	Protein_163	transposase	-
COG34_RS26750	138744..139441	-	698	Protein_164	IS1 family transposase	-
COG34_RS26755	139474..139794	+	321	WP_122993797.1	hypothetical protein	-
COG34_RS26760	140259..140507	+	249	WP_000513531.1	type II toxin-antitoxin system HicA family toxin	-
COG34_RS26765	140504..140851	+	348	WP_000681610.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cfaA / csaC / csaE / etpB / etpA	1..152012	152012
	-	inside	IScluster/Tn	-	-	134184..143298	9114

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15059.51 Da
• Isoelectric Point: 7.7814

>T87252 WP_001356337.1 NZ_CP024244:135711-136127 [Escherichia coli O128:H27]
MKKTYMLDTCICSFIMREQPEAVLNRLEQTVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLIDAFCARLDAVLPWDRT
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTSNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T87252 NZ_CP024244:135711-136127 [Escherichia coli O128:H27]
ATGAAGAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCAGAAGCTGTGCTGAATCGCCT
GGAGCAGACGGTGCTGCGCGGTCACCGTATCGTGGTCTCAGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAGCCTCACCGCGTCACGTACAGTTGATTGACGCGTTCTGCGCCCGGCTCGATGCCGTCCTGCCCTGGGACCGCACC
GCGGTGGATGCCACCACAGAGATTAAGGTGGCCCTGCGCCTGGCCGGCACGCCGATCGGTCCGAACGACACCGCCATTGC
CGGACACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAGTAATGTGAGAGAGTTTGCACGGGTGCCAGGCCTGGTGCTGG
AAGACTGGGTTAACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8792.91 Da
• Isoelectric Point: 4.4992

>AT87252 WP_024220872.1 NZ_CP024244:135484-135714 [Escherichia coli O128:H27]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPVRPSWGSFAQLEKADPDFMAERDDVVTDEGRFEL

Nucleotide

• Length: 231 bp

>AT87252 NZ_CP024244:135484-135714 [Escherichia coli O128:H27]
ATGAGAACAGTATCCATTTTTAAAAACGGCAATAACCGTGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATCGTCCGGGAAGGGGACCGCATCATCCTGCGTCCCGTCCGGCCGAGCTGGGGCTCGTTCGCGCAGCTTG
AAAAGGCCGATCCAGACTTTATGGCAGAGCGCGATGACGTTGTCACCGACGAAGGACGCTTTGAACTATGA