Detailed information of TA system TA087239

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4078634..4079466
• Replicon: chromosome
• Accession: NZ_CP024243
• Organism: Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: COG34_RS21080
• Protein ID: WP_000854762.1
• Coordinates: 4078634..4079008 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: COG34_RS21085
• Protein ID: WP_001295723.1
• Coordinates: 4079098..4079466 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
COG34_RS21050	4074115..4074732	-	618	WP_087603314.1	LuxR family transcriptional regulator	-
COG34_RS21055	4074729..4075190	-	462	WP_071596603.1	CaiF/GrlA family transcriptional regulator	-
COG34_RS21060	4075617..4075910	-	294	Protein_3825	tyrosine-type recombinase/integrase	-
COG34_RS21065	4075940..4076769	-	830	Protein_3826	IS3 family transposase	-
COG34_RS21070	4076819..4078184	+	1366	Protein_3827	IS66-like element ISEc23 family transposase	-
COG34_RS27045	4078305..4078418	-	114	WP_001161660.1	DUF957 domain-containing protein	-
COG34_RS21075	4078431..4078637	-	207	WP_000976829.1	hypothetical protein	-
COG34_RS21080	4078634..4079008	-	375	WP_000854762.1	TA system toxin CbtA family protein	Toxin
COG34_RS21085	4079098..4079466	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
COG34_RS21090	4079629..4079850	-	222	WP_000692341.1	DUF987 domain-containing protein	-
COG34_RS21095	4079913..4080389	-	477	WP_001186774.1	RadC family protein	-
COG34_RS21100	4080405..4080878	-	474	WP_000855059.1	antirestriction protein	-
COG34_RS21110	4081220..4082038	-	819	WP_001234719.1	DUF945 domain-containing protein	-
COG34_RS21120	4082193..4082351	-	159	WP_001433433.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14094.15 Da
• Isoelectric Point: 7.1881

>T87239 WP_000854762.1 NZ_CP024243:c4079008-4078634 [Escherichia coli O128:H27]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T87239 NZ_CP024243:c4079008-4078634 [Escherichia coli O128:H27]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT87239 WP_001295723.1 NZ_CP024243:c4079466-4079098 [Escherichia coli O128:H27]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT87239 NZ_CP024243:c4079466-4079098 [Escherichia coli O128:H27]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: S1NM52