Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4048921..4049423 Replicon chromosome
Accession NZ_CP024243
Organism Escherichia coli O128:H27 strain 90-9281

Toxin (Protein)


Gene name yoeB Uniprot ID -
Locus tag COG34_RS20915 Protein ID WP_000767835.1
Coordinates 4049169..4049423 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag COG34_RS20910 Protein ID WP_001259255.1
Coordinates 4048921..4049172 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
COG34_RS20880 4044098..4045165 - 1068 WP_000080072.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
COG34_RS20885 4045165..4046235 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
COG34_RS20890 4046232..4047536 - 1305 WP_001356284.1 histidinol dehydrogenase -
COG34_RS20895 4047542..4048441 - 900 WP_000131774.1 ATP phosphoribosyltransferase -
COG34_RS20905 4048587..4048637 - 51 WP_001364200.1 his operon leader peptide -
COG34_RS20910 4048921..4049172 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
COG34_RS20915 4049169..4049423 + 255 WP_000767835.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
COG34_RS20920 4049506..4050330 + 825 WP_000754737.1 SDR family oxidoreductase -
COG34_RS20925 4050376..4051305 + 930 WP_000803341.1 LysR family transcriptional regulator -
COG34_RS20930 4051520..4051582 + 63 WP_010723108.1 membrane protein YoeI -
COG34_RS20935 4051572..4052930 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
COG34_RS20940 4053109..4054167 + 1059 WP_000492320.1 transport protein YeeE -
COG34_RS20945 4054181..4054408 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.51 Da        Isoelectric Point: 7.2751

>T87238 WP_000767835.1 NZ_CP024243:4049169-4049423 [Escherichia coli O128:H27]
VKLIWSEESWDDYLYWQETDNRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T87238 NZ_CP024243:4049169-4049423 [Escherichia coli O128:H27]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT87238 WP_001259255.1 NZ_CP024243:4048921-4049172 [Escherichia coli O128:H27]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT87238 NZ_CP024243:4048921-4049172 [Escherichia coli O128:H27]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References